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Results: 3

1.
FIGURE 2

FIGURE 2. From: Cross Talk Free Fluorescence Cross Correlation Spectroscopy in Live Cells.

(A) Pulsed excitation scheme for cross talk free FCCS. Fluorescence time intervals for data analysis can be selected by software. (B) Schematic overview of the FCCS setup.

Elmar Thews, et al. Biophys J. 2005 September;89(3):2069-2076.
2.
FIGURE 1

FIGURE 1. From: Cross Talk Free Fluorescence Cross Correlation Spectroscopy in Live Cells.

Excitation and emission spectra of ECFP and EYFP (from Cubitt et al. (41)). In the shaded area are the excitation wavelengths chosen to prevent cross talk and the undetected spectral range due to the trichroic beamsplitter. The circle stresses the spectral region in which, normally, cross talk arises.

Elmar Thews, et al. Biophys J. 2005 September;89(3):2069-2076.
3.
FIGURE 3

FIGURE 3. From: Cross Talk Free Fluorescence Cross Correlation Spectroscopy in Live Cells.

(A) Histogram of the photon arrival times. Two main peaks corresponding to photons originating from ECFP (left) and EYFP (right) are observable. (B) Background corrected FCS curves of ECFP, EYFP, and the FCCS curve for a mixture of both fluorophores in solution (cyan, CFP autocorrelation; yellow, YFP autocorrelation; black, cross correlation; red, fit functions). (C and D) FCS curves of ECFP, EYFP, and the FCCS curve for the ECFP/EYFP chimera in solution. (C) Measured curves. (D) Background corrected curves. (E and F) FCS curves of ECFP, EYFP, and the FCCS curve for coexpressed ECFP/Cx46 and EYFP/Cx46 in live HeLa cells. (E) Measured curves. (F) Background and permutation corrected curves. (Inset) Schematic top view of six connexins fused to three ECFPs' respective EYFPs.

Elmar Thews, et al. Biophys J. 2005 September;89(3):2069-2076.

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