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Results: 4

1.
Figure 4

Figure 4. From: Replacement of K-Ras with H-Ras supports normal embryonic development despite inducing cardiovascular pathology in adult mice.

BP measurements. (A) Systolic BP evaluated non-invasively by tail-cuff measurements in conscious mice (n=6 for each group). *P<0.01 versus wild type (+/+) and KrasKI. Data are mean±s.e.m. (B) Systolic and diastolic BP evaluated invasively by radiotelemetry in conscious mice (n=3 for each group). *P<0.02 versus +/+ and KrasKI. Data are mean±s.e.m.

Nicoletta Potenza, et al. EMBO Rep. 2005 May;6(5):432-437.
2.
Figure 2

Figure 2. From: Replacement of K-Ras with H-Ras supports normal embryonic development despite inducing cardiovascular pathology in adult mice.

Expression of the Ras isoforms in different tissues from wild-type (WT), KrasKI and HrasKI adult mice. The protein extracts were probed with antibodies specific for each Ras protein. Pan-Ras antibody was used to detect the total expression levels of Ras proteins. Tubulin antibody was used to normalize the amount of loaded proteins.

Nicoletta Potenza, et al. EMBO Rep. 2005 May;6(5):432-437.
3.
Figure 3

Figure 3. From: Replacement of K-Ras with H-Ras supports normal embryonic development despite inducing cardiovascular pathology in adult mice.

Left ventricular remodelling. (A) Representative M-mode left ventricular echocardiographic recordings of wild-type (+/+), KrasKI and HrasKI mice. All measurements were determined in a short-axis view at the level of papillary muscles. (B) Collagen deposition (light blue), indicative of fibrosis, evaluated by Masson's trichrome staining in the left ventricle. (C) Left ventricles processed for histology to measure the cardiomyocyte crosssectional area from +/+, KrasKI and HrasKI mice. (D) Mean values from 200 cardiomyocyte area determinations per mouse. *P<0.05 versus +/+.

Nicoletta Potenza, et al. EMBO Rep. 2005 May;6(5):432-437.
4.
Figure 1

Figure 1. From: Replacement of K-Ras with H-Ras supports normal embryonic development despite inducing cardiovascular pathology in adult mice.

Targeting strategy of knock-in of H-ras (or K-ras) cDNA in K-ras locus. (A) Wild-type K-ras targeted region with targeting vector and the predicted mutant locus. The coding exons are represented by open boxes. The HSV-TK (herpes simplex virus thymidine kinase) and neo cassettes are indicated. The cloned coding sequences for H- or K-ras are represented by dashed boxes. The 3′ untranslated region is represented by a grey box. The probes used for Southern hybridization are shown by black boxes and the expected sizes of the signals are indicated by dashed lines. EI, EcoRI; EV, EcoRV; S, SacI; X, XhoI. (B) Southern blot analysis of genomic DNA from ES clones using 3′ (left) and 5′ (right) probes. +/+, wild type; +/−, HrasKI heterozygous.

Nicoletta Potenza, et al. EMBO Rep. 2005 May;6(5):432-437.

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