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1.
FIG. 4.

FIG. 4. From: Saccharomyces cerevisiae Sps1p Regulates Trafficking of Enzymes Required for Spore Wall Synthesis.

Chs3p-GFP and Gsc2p-GFP fail to reach the prospore membrane in sps1 mutants. Chs3p-GFP localization is shown in living wild-type (wt) and sps1 cells and with the addition of latA (+latA). Gsc2p-GFP (Fks2p-GFP) localization is shown in living wt and sps1 cells.

Michelle A. Iwamoto, et al. Eukaryot Cell. 2005 March;4(3):536-544.
2.
FIG. 6.

FIG. 6. From: Saccharomyces cerevisiae Sps1p Regulates Trafficking of Enzymes Required for Spore Wall Synthesis.

Shc1p-GFP is not properly localized in sps1 mutants but is not required for Chs3p-GFP localization to the prospore membrane. (A) Shc1p-GFP localization in wild-type (wt) and sps1 cells; (B) Chs3p-GFP localization in shc1Δ cells.

Michelle A. Iwamoto, et al. Eukaryot Cell. 2005 March;4(3):536-544.
3.
FIG. 5.

FIG. 5. From: Saccharomyces cerevisiae Sps1p Regulates Trafficking of Enzymes Required for Spore Wall Synthesis.

sps1 mutants are proficient for endocytosis. Wild-type (wt) cells and sps1 mutants stained with FM4-64 are shown. Numbers above the panels refer to time (in minutes) after FM4-64 was washed out.

Michelle A. Iwamoto, et al. Eukaryot Cell. 2005 March;4(3):536-544.
4.
FIG. 7.

FIG. 7. From: Saccharomyces cerevisiae Sps1p Regulates Trafficking of Enzymes Required for Spore Wall Synthesis.

GFP-Sps1p localizes to internal structures and prospores and displays colocalization with Chs3p-dsRed. Left (green), GFP-Sps1p localization in living cells induced in sporulation; center (red), Chs3p-dsRed localization in living cells induced in sporulation; right (yellow), merged image showing colocalization of GFP-Sps1p and Chs3p-dsRed.

Michelle A. Iwamoto, et al. Eukaryot Cell. 2005 March;4(3):536-544.
5.
FIG. 3.

FIG. 3. From: Saccharomyces cerevisiae Sps1p Regulates Trafficking of Enzymes Required for Spore Wall Synthesis.

Dityrosine is synthesized but not incorporated into the spore wall in sps1 mutants. Extracts were prepared and fluorescence was measured as described in Materials and Methods. The intensity of emission was measured at 420 nm. The peak at 315 nm corresponds to dityrosine.

Michelle A. Iwamoto, et al. Eukaryot Cell. 2005 March;4(3):536-544.
6.
FIG. 1.

FIG. 1. From: Saccharomyces cerevisiae Sps1p Regulates Trafficking of Enzymes Required for Spore Wall Synthesis.

Prospore membrane formation appears normal in sps1 mutants. (A) GFP-Spo14p staining in the wild type (wt) and sps1 mutants; (B) Don1p-GFP staining in the wt and sps1 and spo14Δ mutants. (C) Spr28p-GFP staining in the wt and sps1 mutants; (D) Dtr1p-GFP staining in the wt and sps1 mutants. Living cells were visualized at the time of the meiotic divisions.

Michelle A. Iwamoto, et al. Eukaryot Cell. 2005 March;4(3):536-544.
7.
FIG. 2.

FIG. 2. From: Saccharomyces cerevisiae Sps1p Regulates Trafficking of Enzymes Required for Spore Wall Synthesis.

sps1 mutants do not display the full array of spore wall layers. The left panels show wild-type (wt) cells with characteristic staining patterns of calcofluor white (chitosan), anti-glucan antibodies (β-1,3-glucan), and FITC-ConA (mannan) around each individual spore. The fourth spore of the tetrad is out of the plane of focus. sps1 mutants do not display staining with calcofluor white or anti-glucan antibodies around each spore-like structure but do have distinct FITC-ConA staining.

Michelle A. Iwamoto, et al. Eukaryot Cell. 2005 March;4(3):536-544.

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