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Results: 4

1.
FIG. 1.

FIG. 1. From: SYBR Green-Based Real-Time Quantitative PCR Assay for Detection of West Nile Virus Circumvents False-Negative Results Due to Strain Variability.

Linearity and sensitivity comparisons of TaqMan and SYBR green-based real-time quantitative PCRs. Linear regression plots were generated for TaqMan and SYBR green-based real-time quantitative PCRs of WN-NY99 dilutions with the same WNV env primer set. The sample dilution on the horizontal axis is plotted against the CT on the vertical axis (n = 3). (A) Linear regression of TaqMan assay. (B) Linear regression of SYBR green-based assay.

James F. Papin, et al. J Clin Microbiol. 2004 April;42(4):1511-1518.
2.
FIG. 4.

FIG. 4. From: SYBR Green-Based Real-Time Quantitative PCR Assay for Detection of West Nile Virus Circumvents False-Negative Results Due to Strain Variability.

Titration of WNV from an avian sample in a Vero cell culture. (A) Plot of amplification of serially diluted supernatants from infected Vero cell cultures. The cycle number on the horizontal axis is plotted against the relative measured fluorescence (Rn) on the vertical axis. (B) Dissociation plot of amplification products. The temperature on the horizontal axis is plotted against the derivative of the measured fluorescence [d(F)] on the vertical axis.

James F. Papin, et al. J Clin Microbiol. 2004 April;42(4):1511-1518.
3.
FIG. 3.

FIG. 3. From: SYBR Green-Based Real-Time Quantitative PCR Assay for Detection of West Nile Virus Circumvents False-Negative Results Due to Strain Variability.

Comparison of the effects of nucleotide variations on the TaqMan and SYBR green-based assays. (A) Plot of amplification of 96 mutant oligonucleotides in the SYBR green-based assay. The cycle number on the horizontal axis is plotted against the relative measured fluorescence (Rn) on the vertical axis. (B) CT values obtained for individual mutant target oligonucleotides in either the SYBR green-based assay (horizontal axis) or the TaqMan assay (vertical axis). Samples that did not yield any signal were assigned a CT value of 45. (C) Agarose (2%) gel electrophoresis of the amplification products from the TaqMan assay stained with ethidium bromide. The asterisk indicates an empty lane. (D) Dissociation plot of 96 identical samples (WN-NY99) after amplification in the SYBR green-based assay with the WNV env primer set. (E) Dissociation plot of the 96 mutant oligonucleotides listed in Table 1 after amplification in the SYBR green based assay with the WNV env primer set. The derivative of the measured fluorescence [d(F)] on the vertical axis is plotted against the temperature at which it was sampled on the horizontal axis for panels D and E.

James F. Papin, et al. J Clin Microbiol. 2004 April;42(4):1511-1518.
4.
FIG. 2.

FIG. 2. From: SYBR Green-Based Real-Time Quantitative PCR Assay for Detection of West Nile Virus Circumvents False-Negative Results Due to Strain Variability.

Detection of WN virus in mosquito pools by SYBR green-based real-time RT-QPCR. (A) Plot of amplification of mosquito pools with the WNV env primer set. Cycle number on the horizontal axis is plotted against the relative fluorescence (Rn) on the vertical axis (log scale). Dotted lines indicate the two positive control reactions. Positive and negative mosquito pools are indicated by plus and minus signs, respectively. (B) Agarose (2%) gel electrophoresis of amplification products stained with ethidium bromide. Lane M, molecular weight markers. Lanes +, positive control (in duplicate). Positive reactions showed a single amplification product of approximately 60 bp. (C) Dissociation plot of amplification products for the positive control and mosquito pools. Temperature on the x axis is plotted against the first derivative of the measured fluorescence [d(F)] on the y axis. The asterisk indicates the peak position of the reference dissociation curve.

James F. Papin, et al. J Clin Microbiol. 2004 April;42(4):1511-1518.

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