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Results: 4

1.
Fig. 1.

Fig. 1. From: Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates.

Cloverleaf representation of A. ferrooxidans tRNAGlu and tRNAGln species. The bases that form part of the augmented D-helix (13), in both isoacceptors of tRNAGlu and in , are shown in white letters and black shadow.

Juan C. Salazar, et al. Proc Natl Acad Sci U S A. 2003 November 25;100(24):13863-13868.
2.
Fig. 3.

Fig. 3. From: Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates.

Aminoacylation of tRNAGlu and tRNAGln from E. coli and A. ferrooxidans. The reaction conditions were as indicated in Materials and Methods by using the pure tRNAs. (A) E. coli tRNAGlu.(B) E. coli tRNAGln.(C) A. ferrooxidans . (D) A. ferrooxidans . The enzymes used were E. coli GluRS (▿) and GlnRS (Δ), H. pylori GluRS1 (•) and GluRS2 (▪), and A. ferrooxidans GluRS1 (ο) and GluRS2 (□). Glutamic acid was used with the GluRS, and glutamine was used with GlnRS.

Juan C. Salazar, et al. Proc Natl Acad Sci U S A. 2003 November 25;100(24):13863-13868.
3.
Fig. 2.

Fig. 2. From: Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates.

Phylogeny of bacterial-type GluRS proteins. Amino acid sequences were aligned by using the clustalw program (Version 1.82) (28). Initial analyses were performed with GluRS proteins from numerous bacterial organisms, and later the same was confirmed with a set of representative GluRSs. Phylogeny was inferred by using the neighbor-joining method to create and evaluate 1,000 resampled alignments. Bootstrap percentages are given for each branch. (Bar = 10 aa replacements per 100 positions.)

Juan C. Salazar, et al. Proc Natl Acad Sci U S A. 2003 November 25;100(24):13863-13868.
4.
Fig. 4.

Fig. 4. From: Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates.

Northern blot analysis of H. pylori and A. ferrooxidans tRNA after in vitro acylation by the different GluRS enzymes. The blots were probed with 32P-labeled oligonucleotide complementary to the anticodon stem or plus acceptor stem (see Materials and Methods). Blots correspond to the detection of tRNAGln (Left) and to tRNAGlu (Right). (A) Unfractionated tRNA from H. pylori. (B) Unfractionated tRNA from A. ferrooxidans probed for or . (C) Unfractionated tRNA from A. ferrooxidans probed for or . Lane 1, charging of unfractionated tRNA with glutamine by E. coli GlnRS. The enzymes used are: E1, GluRS1; E2, GluRS2; Q, E. coli GlnRS. The arrows indicate positions of AA-tRNA: a, uncharged tRNAGln; b, Glu-tRNAGln; c, Gln-tRNAGln; d, uncharged tRNAGlu; and e, Glu-tRNAGlu.

Juan C. Salazar, et al. Proc Natl Acad Sci U S A. 2003 November 25;100(24):13863-13868.

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