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Results: 5

1.
Fig. 2.

Fig. 2. From: RNA interference-mediated knockdown of a GATA factor reveals a link to anautogeny in the mosquito Aedes aegypti.

(A) Schematic drawing of AaGATAr RNAi construct. (B) Schematic representation of the AaGATAr RNAi Sindbis virus and subgenomic RNAs expressed during infection. CAP, mRNA 5′ Cap Structure.

Geoffrey M. Attardo, et al. Proc Natl Acad Sci U S A. 2003 November 11;100(23):13374-13379.
2.
Fig. 1.

Fig. 1. From: RNA interference-mediated knockdown of a GATA factor reveals a link to anautogeny in the mosquito Aedes aegypti.

AaGATAr mRNA expression profile. Real-time PCR analysis was performed on cDNA samples obtained from mosquito fat bodies dissected at 12-h intervals during the previtellogenic state and either 2- or 4-h intervals during the vitellogenic state. cDNA was synthesized from total RNA from groups of five fat bodies per time point. Reactions were performed in triplicate. Data represent means ± SEM of triplicate samples.

Geoffrey M. Attardo, et al. Proc Natl Acad Sci U S A. 2003 November 11;100(23):13374-13379.
3.
Fig. 3.

Fig. 3. From: RNA interference-mediated knockdown of a GATA factor reveals a link to anautogeny in the mosquito Aedes aegypti.

Expression of the RNAi construct subgenomic RNA by Sindbis virus in infected mosquitoes. Five- to 7-day-old mosquitoes were infected with either control Sindbis virus (lacking insert) or Sindbis virus carrying the AaGATAr RNAi construct and allowed to incubate for 5 days at 28°C, 80% relative humidity. Wild-type mosquitoes are uninfected. Total RNA (7.5 μg) from each sample group was blotted and analyzed by Northern blotting by using a probe against the AaGATAr fragment used in the RNAi construct.

Geoffrey M. Attardo, et al. Proc Natl Acad Sci U S A. 2003 November 11;100(23):13374-13379.
4.
Fig. 5.

Fig. 5. From: RNA interference-mediated knockdown of a GATA factor reveals a link to anautogeny in the mosquito Aedes aegypti.

The Vg gene is sensitized to the presence of 20E in AaGATAr knockdown mosquitoes. Five- to 7-day-old mosquitoes were infected with either control Sindbis virus (lacking insert) or Sindbis virus carrying the AaGATAr RNAi construct and allowed to incubate for 5 days at 28°C, 80% relative humidity. No virus treatments are uninfected wild-type mosquitoes. After 5 days, mosquitoes were injected with 0.5 μl of either ethanol (control) or 10-6 M 20E. Vg expression was quantified by real-time PCR as described in Fig. 4B. Data represent means ± SEM of triplicate samples.

Geoffrey M. Attardo, et al. Proc Natl Acad Sci U S A. 2003 November 11;100(23):13374-13379.
5.
Fig. 4.

Fig. 4. From: RNA interference-mediated knockdown of a GATA factor reveals a link to anautogeny in the mosquito Aedes aegypti.

Vitellogenin expression is derepressed in AaGATAr RNAi-treated mosquitoes. Five- to 7-day-old mosquitoes were infected with either control Sindbis virus (lacking insert) or Sindbis virus carrying the AaGATAr RNAi construct and allowed to incubate for 5-6 days at 28°C, 80% relative humidity. Wild-type mosquitoes are uninfected. (A) Northern blot analysis of Vg expression in AaGATAr RNAi mosquitoes. Total RNA (7.5 μg) from each sample group was blotted and analyzed by Northern blotting by using a probe against vitellogenin. (B) Real-time PCR analysis of Vg expression in RNAi-treated mosquitoes. cDNA was synthesized from total RNA from groups of five fat bodies per time point. Reactions were performed in triplicate. Data were normalized by real-time PCR analysis of Actin levels in the cDNA samples. Data represent means ± SEM of triplicate samples. (C) Western blot analysis of Vg expression in AaGATAr RNAi mosquitoes. Total protein was extracted from groups of five mosquitoes. Twenty micrograms of total protein was blotted and probed with a monoclonal antibody against the 68-kDa small subunit of Vg.(D) Real-time PCR analysis of AaGATAr expression in RNAi-treated mosquitoes. Analysis was performed as in B. Data represent means ± SEM of triplicate samples.

Geoffrey M. Attardo, et al. Proc Natl Acad Sci U S A. 2003 November 11;100(23):13374-13379.

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