Results: 3

1.
Fig. 1.

Fig. 1. From: Expression of T cell receptor ?locus in central nervous system neurons.

TCRβ mRNA is expressed in the mouse brain. Cryostat sections from postnatal days 0, 4, 7, 28, or 40 were either stained with cresyl violet or hybridized with a probe for TCRβ C regions. (A) Coronal sections. (B) P40 TCRβ-/- and TCRβ wild-type sense control sections are similar to the P40 section hybridized with TCRβ antisense probe. (C) Sagittal sections, anterior to the left. TH, thalamus; AG, amygdala; dLGN, dorsal lateral geniculate nucleus; vLGN, ventral lateral geniculate nucleus; CTX, cortex. (Scale bars, 1 mm.)

Josh Syken, et al. Proc Natl Acad Sci U S A. 2003 October 28;100(22):13048-13053.
2.
Fig. 2.

Fig. 2. From: Expression of T cell receptor ?locus in central nervous system neurons.

TCRβ is expressed in a subset of cortical neurons. (A and B) P40 coronal section showing cortical layers 1–6 (A) and TCRβ C region in situ hybridization shown in darkfield optics (B) (silver grains appear white). (C) High-power image of layer 6 cortical cells double-stained with cresyl violet (purple) and TCRβ in situ hybridization (black silver grains). Arrows point to large pale nuclei with associated silver grains; asterisks indicate smaller dark nuclei. (D) Triple labeling of cortical layer 6 neurons from posterior cortical region. Hoechst-stained nuclei (blue), retrograde label from Fluoro-Gold injection in striatum (green), and TCRβ in situ hybridization (red) are shown. Arrow indicates triple-labeled cell; asterisk indicates nucleus of a cell with no retrograde label and no TCRβ expression.

Josh Syken, et al. Proc Natl Acad Sci U S A. 2003 October 28;100(22):13048-13053.
3.
Fig. 3.

Fig. 3. From: Expression of T cell receptor ?locus in central nervous system neurons.

Neuronal TCRβ is transcribed from unrearranged genomic loci. (A Top) Northern blot analysis of RNA derived from P9 thalamus (Tha), P29 cerebellum (Cb), P30 cortex (Ctx), and adult spleen (Spl), probed with TCRβ C region or mix of V region probes. (Bottom) RNA derived from P30, P40, P50, and P74 cortex, adult spleen, P6 and P40 thalamus, and P40 and P50 cerebellum, probed for TCRβ C region. M, molecular weight markers. (B) RACE-derived sequences indicate that the 5′ ends of neuronal TCRβ are identical with genomic sequences 5′ to several of the TCRβ J regions. Numbers indicate bases from the beginning of transcript to Jβ exon junction. (C) In situ hybridization of adult mouse brain sections by using probes for genomic sequence spanning Jβ1.1–1.6 or Jβ2.1–2.7 indicates that the majority of neuronal TCRβ mRNA includes Jβ2 regions. (D) RT-PCR by using primers ≈20 bp 5′ to the start of each Jβ coding region indicated, plus a common C region 3′ primer, indicate that neuronal TCRβ transcripts include at least 8 of the 12 Jβ regions and include noncoding intergenic sequences upstream of Jβ regions. One sequence that included Jβ1.2 also included the Cβ0 exon. (E) Hypothetical truncated TCRβ protein sequence. (Top) Amino acid sequence of hypothetical protein encoded by genomic region 5′ to Jβ2.7 (blue), Jβ2.7 (green), and C region (black). (Bottom) Immunoprecipitation/Western blot analysis of saline-perfused brain tissue using an antibody to the C region of TCRβ. LC, light chain; Sp, spleen; Ctx, cortex; Dcph, diencephalon. Analyzed were 100 μg of spleen tissue and 5 mg of cortex and diencephalon.

Josh Syken, et al. Proc Natl Acad Sci U S A. 2003 October 28;100(22):13048-13053.

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