Results: 5

1.
FIG. 4.

FIG. 4. From: Genotyping and Phenotyping of Beta2-Toxigenic Clostridium perfringens Fecal Isolates Associated with Gastrointestinal Diseases in Piglets.

Analysis of clonal relationships among C. perfringens pig GI disease isolates. DNA from each of the specified C. perfringens isolates in agarose plugs was digested with SmaI (A) or MluI (B) and subjected to PFGE and ethidium bromide straining. The gel was calibrated with bacteriophage lambda ladder DNA. The molecular sizes of the DNA markers are between the two gels.

Michael Waters, et al. J Clin Microbiol. 2003 August;41(8):3584-3591.
2.
FIG. 2.

FIG. 2. From: Genotyping and Phenotyping of Beta2-Toxigenic Clostridium perfringens Fecal Isolates Associated with Gastrointestinal Diseases in Piglets.

RFLP and Southern blotting analyses of HpaI- or EcoRV-digested DNA from pig GI disease isolates. Total DNA isolated from each of the specified C. perfringens strains was digested with HpaI (A) or EcoRV (B) and then Southern transferred. The Southern blots were probed with a 318-bp, DIG-labeled cpb2-specific probe. Results are shown for control strains CWC245 (a known cpb2-positive type C isolate) and 106527 (a cpb2-negative type C strain) and representative cpb2-positive pig GI disease isolates JGS1538, 120-98, 170-98, JGS1548, 97-4029, 2011, and JGS1818. The migration of the hybridizing band derived from each strain is indicated between the two blots.

Michael Waters, et al. J Clin Microbiol. 2003 August;41(8):3584-3591.
3.
FIG. 3.

FIG. 3. From: Genotyping and Phenotyping of Beta2-Toxigenic Clostridium perfringens Fecal Isolates Associated with Gastrointestinal Diseases in Piglets.

PFGE evidence supporting the plasmid localization of cpb2 in pig GI disease isolates. PFGE and Southern hybridization were used to analyze undigested DNA, prepared in agarose plugs, from each of the C. perfringens isolates specified. The blots were probed with a 318-bp cpb2-specific probe. Results are shown for control strains CWC245 (a cpb2-positive type C isolate carrying the cpb2 gene on a large plasmid) and 106527 (a cpb2-negative type C isolate) and representative cpb2 pig isolates JGS1552, JGS1561, JGS1817, 2011, 2142, and 10618J. The pulsed-field gel was calibrated with bacteriophage lambda DNA markers, whose migration is shown at the left of the blot.

Michael Waters, et al. J Clin Microbiol. 2003 August;41(8):3584-3591.
4.
FIG. 1.

FIG. 1. From: Genotyping and Phenotyping of Beta2-Toxigenic Clostridium perfringens Fecal Isolates Associated with Gastrointestinal Diseases in Piglets.

PCR analysis of pig GI disease isolates. (A) Representative results of a PCR assay with primers designed to amplify 1,373- and 318-bp PCR products. The migration of the PCR products derived from each primer set is indicated between the two gels. Results are shown for control strains CWC245 (a known cpb2-positive C. perfringens type C strain) and 106527 (a cpb2-negative type C strain) and representative pig GI disease isolates 120-98, 170-98, and JGS1817. DNA size markers (GeneRuler 1-kb ladder; Fermentas) are shown in the left lane of each gel. (B) Schematic diagram showing the location of each primer in CWC245 DNA ().

Michael Waters, et al. J Clin Microbiol. 2003 August;41(8):3584-3591.
5.
FIG. 5.

FIG. 5. From: Genotyping and Phenotyping of Beta2-Toxigenic Clostridium perfringens Fecal Isolates Associated with Gastrointestinal Diseases in Piglets.

Western blotting analysis of CPB2 expression by selected pig GI disease isolates. (A) Culture supernatant proteins (sup) or total cell (cell) proteins, prepared from each of the C. perfringens isolates specified, were separated by SDS-polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue. The migrations of the supernatant proteins are shown by arrows. (B) Western blot of the gel shown in panel A. The blot was probed with CPB2 antibodies and developed by chemiluminescence detection to identify immunoreactive species. Results are shown for control strains CWC245 (a cpb2-positive type C strain) and 106527 (a cpb2-negative type C strain) and representative pig GI disease isolates JGS1807 and 106640. Molecular mass markers (in kilodaltons) are shown between the gels.

Michael Waters, et al. J Clin Microbiol. 2003 August;41(8):3584-3591.

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