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1.
Figure 3.

Figure 3. From: Two Newly Characterized Germinal Center B-Cell-Associated Genes, GCET1 and GCET2, Have Differential Expression in Normal and Neoplastic B Cells.

A: Locations of GCET2 and adjacent genes on chromosome 3q13.13. B: Northern blot hybridization of GCET2. C: Nucleotide and protein sequence data of GCET2. Bolded nucleotides are the possible elements for polyadenylation.

Zenggang Pan, et al. Am J Pathol. 2003 July;163(1):135-144.
2.
Figure 5.

Figure 5. From: Two Newly Characterized Germinal Center B-Cell-Associated Genes, GCET1 and GCET2, Have Differential Expression in Normal and Neoplastic B Cells.

A: Top, Standard amplification plot for reference gene, β actin (ACTB); bottom, ACTB standard curve. The serial dilutions of the standard are expressed in nature log values in the y axis. B: Top, Standard amplification plot for GCET1; bottom, GCET1 standard curve. C: Top, Standard amplification plot for GCET2; bottom, GCET2 standard curve. Standard amplification plots and curves for B2M and GAPDH were similar (not shown).

Zenggang Pan, et al. Am J Pathol. 2003 July;163(1):135-144.
3.
Figure 4.

Figure 4. From: Two Newly Characterized Germinal Center B-Cell-Associated Genes, GCET1 and GCET2, Have Differential Expression in Normal and Neoplastic B Cells.

A: Sequence alignment between human GCET2 and AAH24174. The homology is 34% (46 of 135) (does not include positively charged amino acid residues). B: Homology between GCET2 and mouse M17 is 57% (89 of 157) (does not include positively charged amino acid residues). Bolded amino acid sequences are the possible conserved tyrosine phosphorylation sites.

Zenggang Pan, et al. Am J Pathol. 2003 July;163(1):135-144.
4.
Figure 7.

Figure 7. From: Two Newly Characterized Germinal Center B-Cell-Associated Genes, GCET1 and GCET2, Have Differential Expression in Normal and Neoplastic B Cells.

Comparison of real-time PCR with microarray data for GCET1 and GCET2. All of the real-time PCR data were multiplied by 16 and converted into log2 values, with the value of DHL16 standard set at 4 to obtain a better spread of the colors. These values were converted into pseudocolors with green representing low expression and red representing high expression according to the predefined scheme shown. R, Real-time PCR data; M, microarray data.

Zenggang Pan, et al. Am J Pathol. 2003 July;163(1):135-144.
5.
Figure 1.

Figure 1. From: Two Newly Characterized Germinal Center B-Cell-Associated Genes, GCET1 and GCET2, Have Differential Expression in Normal and Neoplastic B Cells.

Microarray gene expression profiles of GCET1 and GCET2. A: Both GCET1 and GCET2 are highly expressed in normal GC B cells (a) and FL (f); down-regulated in activated and resting peripheral B cells (b, g), resting and activated T cells (c, e), and CLL (h). B: They have high expression levels in GCB-DLBCL but low expression in the ABC group.

Zenggang Pan, et al. Am J Pathol. 2003 July;163(1):135-144.
6.
Figure 6.

Figure 6. From: Two Newly Characterized Germinal Center B-Cell-Associated Genes, GCET1 and GCET2, Have Differential Expression in Normal and Neoplastic B Cells.

Expression profiling of GCET1 and GCET2. A: GCET1 is highly expressed in microdissected normal GCs, significantly lower in MZB, and very low in MB. In normal tonsil and spleen, it has a moderate expression level. B: GCET1 has a high expression in FL and GCB-DLBCL samples, and its expression is significantly lower in CLL and ABC-DLBCL samples. C: Compared with MB and MZB, normal GCs also have a high expression of GCET2. Normal tonsil and spleen express a moderate level of GCET2. GCET2 also has moderately high expression in thymus and Nalm6. D: In FL, CLL, and DLBCL samples, the expression of GCET2 is very similar to that of GCET1 except for one CLL sample. All of the real-time PCR data are expressed as a ratio to DHL16, which is arbitrarily set at 1.

Zenggang Pan, et al. Am J Pathol. 2003 July;163(1):135-144.
7.
Figure 2.

Figure 2. From: Two Newly Characterized Germinal Center B-Cell-Associated Genes, GCET1 and GCET2, Have Differential Expression in Normal and Neoplastic B Cells.

A: 5′ RACE for GCET1 reveals two major bands on agarose gel electrophoresis. B: Distribution of GCET1-splicing isoforms and other known SERPIN family members on chromosome 14q32. C: Northern blot of GCET1 shows a transcript around 1.8 kb in DHL16. D: Alignment of GCET1-splicing isoforms. GCET1A is 1787 bp long encoding a protein of 435 amino acids. GCET1B is 300 bp shorter at the 5′ end encoding a protein of 335 amino acids. GCET1C is 190 bp shorter at the 5′ end encoding a protein of 337 amino acids. GCET1D has 393 bp missing at the 5′ end compared with GCET1A and encodes a protein of 286 amino acids, but it has an extra exon as shown. E: Nucleotide and protein sequence data of GCET1A. Bolded sequence is the conserved SERPIN motif and the underlined nucleotides represent the possible element for polyadenylation.

Zenggang Pan, et al. Am J Pathol. 2003 July;163(1):135-144.

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