We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 4

1.
Figure 3

Figure 3. From: Optimisation of the 10-23 DNAzyme-substrate pairing interactions enhanced RNA cleavage activity at purine-cytosine target sites.

Base pair structures showing hydrogen bond interactions.

Murray J. Cairns, et al. Nucleic Acids Res. 2003 June 1;31(11):2883-2889.
2.
Figure 4

Figure 4. From: Optimisation of the 10-23 DNAzyme-substrate pairing interactions enhanced RNA cleavage activity at purine-cytosine target sites.

Reaction progress curves comparing the activity of rC-dG and rC-dI core DNAzyme–substrate pairs for the substrate series S1. The average rates of these reactions were also recorded in Table 1.

Murray J. Cairns, et al. Nucleic Acids Res. 2003 June 1;31(11):2883-2889.
3.
Figure 2

Figure 2. From: Optimisation of the 10-23 DNAzyme-substrate pairing interactions enhanced RNA cleavage activity at purine-cytosine target sites.

Reaction progress for each RY substrate (S1 series) and their matched DNAzymes. Reaction progress at each time point was determined by densitometry of cleavage band intensity. Data points represented by squares, circles, triangles and rhombi correspond to the GU, AU, GC and AC substrates, respectively. Curves were fitted to the data by regression using the formula described in Materials and Methods. The average rates of these reactions were also recorded in Table 1.

Murray J. Cairns, et al. Nucleic Acids Res. 2003 June 1;31(11):2883-2889.
4.
Figure 1

Figure 1. From: Optimisation of the 10-23 DNAzyme-substrate pairing interactions enhanced RNA cleavage activity at purine-cytosine target sites.

Secondary structure of the 10–23 and 8–17 DNAzyme–substrate complexes. The 10–23 DNAzyme (top) consists of two variable binding domains, designated arm I and arm II, which flank a conserved 15 base unpaired motif that forms the catalytic domain. The only requirement of the RNA polynucleotide substrate is for a core sequence containing an RY junction. The 8–17 DNAzyme (bottom) has a similar configuration to the 10–23 DNAzyme except that it has a 12 base catalytic motif and a core substrate requirement for AG in which the A is unpaired and the G forms a wobble pair.

Murray J. Cairns, et al. Nucleic Acids Res. 2003 June 1;31(11):2883-2889.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk