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Results: 4

1.
Figure 2

Figure 2. From: Transient activation of ?-catenin signaling in cutaneous keratinocytes is sufficient to trigger the active growth phase of the hair cycle in mice.

Growth and differentiation of the hair follicle in K5/S33Yβ-catenin–ER mice treated daily with 4-OHT. A region of dorsal hair was clipped on both transgenic and wild-type littermates and the skin area was treated daily with 4-OHT in ethanol. Parasagittal sections of skin were taken at time points following initiation of treatment and stained with hematoxylin and eosin. Hair follicles in wild-type mice remained in the resting phase (telogen) throughout the experiment; growth and differentiation of transgenic hair follicles was dramatically stimulated.

David Van Mater, et al. Genes Dev. 2003 May 15;17(10):1219-1224.
2.
Figure 3

Figure 3. From: Transient activation of ?-catenin signaling in cutaneous keratinocytes is sufficient to trigger the active growth phase of the hair cycle in mice.

Altered expression of hair follicle markers in K5/S33Yβ-catenin–ER mice. Immunohistochemistry and immunofluorescence were performed on parasagittal sections of skin from both wild-type and K5/S33Yβ-catenin–ER mice in addition to a normal, spontaneous anagen cycle in a C57BL/6J mouse. The markers used were as follows: BrdU (a–c), K17 (d–f), K6 (g–i), K5 (j–l), trichohyalin (m–o), hair keratin (p–r), and alkaline phosphatase staining for dermal papillae (s–u). White arrows highlight ectopic trichohyalin staining in n. Bars: a–l,s–u, 100 μm; m–r, 200 μm.

David Van Mater, et al. Genes Dev. 2003 May 15;17(10):1219-1224.
3.
Figure 4

Figure 4. From: Transient activation of ?-catenin signaling in cutaneous keratinocytes is sufficient to trigger the active growth phase of the hair cycle in mice.

Transient treatment with 4-OHT is sufficient for normal anagen induction in K5/S33Yβ-catenin–ER mice. (A) Hair growth in K5/S33Yβ-catenin–ER mice following a single topical treatment with 4-OHT. The experiment was performed on L2 transgenic mice obtained following backcrossing to C57BL/6J mice for six generations. Hair on wild-type and transgenic littermates was clipped and the skin region treated with a single dose of 4-OHT in ethanol or ethanol alone. Hair growth at 17 d after 4-OHT treatment is shown. (B) Histology of hair follicles in K5/S33Yβ-catenin–ER mice over time after a single treatment with 4-OHT. A cohort of transgenic mice was clipped and treated with 4-OHT and followed for a period of 28 d. Parasagittal skin sections obtained at various times after 4-OHT treatment were stained with hematoxylin and eosin. Bar, 100 μm.

David Van Mater, et al. Genes Dev. 2003 May 15;17(10):1219-1224.
4.
Figure 1

Figure 1. From: Transient activation of ?-catenin signaling in cutaneous keratinocytes is sufficient to trigger the active growth phase of the hair cycle in mice.

Activation of the K5/S33Yβ-catenin–ER protein by 4-OHT and expression in transgenic mice. (A) Schematic of the K5/S33Yβ-catenin–ER construct. A cDNA encoding the chimeric protein was subcloned into a bovine K5 expression cassette. (B) Activity of the K5/S33Yβ-catenin–ER protein is inducible by 4-OHT in 1811 keratinocytes. The 1811 keratinocytes were transfected with an empty K5 cassette, the pBabe/S33Yβ-catenin–ER construct, or the K5/S33Yβ-catenin–ER construct along with either the TCF-responsive reporter construct TOPFLASH or control FOPFLASH construct. Cells were then treated with either 4-OHT in ethanol, or ethanol alone, and harvested 30 h later to assess luciferase activity. The assays were performed in duplicate; data are reported as the ratio of relative light units for TOPFLASH:FOPFLASH, normalized for transfection efficiency. (C) Expression of the K5/S33Yβ-catenin–ER fusion protein relative to endogenous β-catenin in transgenic mouse lines. Protein was isolated from tail skin of F1 mice derived from three independently derived founders and subjected to Western blot analysis with a mouse monoclonal anti-β-catenin antibody. The blot was reprobed with an anti-β-actin antibody to verify equal loading and transfer. (D) β-catenin protein localization in a clipped region of dorsal skin from K5/S33Yβ-catenin–ER transgenic mice not treated (−4-OHT) or 24 h after treatment with a single topical dose of 4-OHT (+4-OHT).

David Van Mater, et al. Genes Dev. 2003 May 15;17(10):1219-1224.

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