Results: 3

1.

FIG. 3. From: Harmonization of Pulsed-Field Gel Electrophoresis Protocols for Epidemiological Typing of Strains of Methicillin-Resistant Staphylococcus aureus: a Single Approach Developed by Consensus in 10 European Laboratories and Its Application for Tracing the Spread of Related Strains.

Dendrogram of all 21 type strains from gels from seven laboratories analyzed in the Laboratory of Hospital Infection, Central Public Health Laboratory, using GelCompar with percent similarity calculated by Dice coefficient (tolerance of 1% and optimization of 0.5%) and represented by UPGMA. Bands larger than 674 kb (as bands above the highest band of the standard will not be normalized) and smaller than 36 kb (the third-lowest band of the standard), as resolution of bands below this is often poor, were not defined for the purpose of further analysis of the gel. Clones A, B, and C are shown (see text for details).

Stephen Murchan, et al. J Clin Microbiol. 2003 April;41(4):1574-1585.
2.
FIG. 2.

FIG. 2. From: Harmonization of Pulsed-Field Gel Electrophoresis Protocols for Epidemiological Typing of Strains of Methicillin-Resistant Staphylococcus aureus: a Single Approach Developed by Consensus in 10 European Laboratories and Its Application for Tracing the Spread of Related Strains.

Four examples of gel images of the 21 representative type strains of European EMRSA strains digested with SmaI and with the final harmonized protocol for PFGE as performed in 9 of the 10 laboratories. NCTC 8325 is the reference standard used to normalize the gels. Bands larger than 674 kb (as bands above the highest band of the standard will not be normalized) and smaller than 36 kb (the third-lowest band of the standard) (as resolution of bands below this is often poor) were not defined for the purpose of further analysis of the gel. (A) Example of a good gel. (B) An underrunning gel due to a lower running temperature. (C) An overloaded gel. (D) An inadequately destained gel. See the text for further details and the legend to Fig. 1 for the running order of the 21 strains.

Stephen Murchan, et al. J Clin Microbiol. 2003 April;41(4):1574-1585.
3.
FIG. 1.

FIG. 1. From: Harmonization of Pulsed-Field Gel Electrophoresis Protocols for Epidemiological Typing of Strains of Methicillin-Resistant Staphylococcus aureus: a Single Approach Developed by Consensus in 10 European Laboratories and Its Application for Tracing the Spread of Related Strains.

Gel image obtained in center 1 following PFGE of representative type strains of European EMRSA by using the initial harmonized protocol and NCTC 8325 as the reference standard. Molecular sizes in kilobases are shown for the reference standard. Lanes 1, 6, 12, 18, 23, and 28, NCTC 8325; lane 2, Belgium E1; lane 3, Belgium E2; lane 4, Belgium E3; lane 5, lambda; lane 7, Finland E1; lane 8, Finland E5; lane 9, Finland E24; lane 10, Finland E10; lane 11, Finland E7; lane 13, France A; lane 14, France B; lane 15, France C; lane 16, Greece 1; lane 17, Spain E1; Lane 19, North German I; lane 20, South German II; lane 21, Hannover III; lane 22, Berlin IV; lane 24, United Kingdom EMRSA-1; lane 25, United Kingdom EMRSA-3; lane 26, United Kingdom EMRSA-15; lane 27, United Kingdom EMRSA-16.

Stephen Murchan, et al. J Clin Microbiol. 2003 April;41(4):1574-1585.

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