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Results: 3

1.
Figure 2

Figure 2. From: Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry.

Time course of chromatographic peak areas of phosphopeptides normalized to LIEDNEpYTAR peak areas. CD3ζ Tyr-141 (♦), CD3δ Tyr-160 (■), CD3ɛ Tyr-199 (●), CD3ζ Tyr-122(+), CD3δ Tyr-149 (−), ZAP-70 Tyr-315,319 (▴), and ZAP-70 Tyr-493 (*). Profiles shown here are representative of all observed.

Arthur R. Salomon, et al. Proc Natl Acad Sci U S A. 2003 January 21;100(2):443-448.
2.
Figure 1

Figure 1. From: Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry.

MS/MS spectrum of the CD3ζ-derived peptide GHDGLpYQGLSTATK. Predicted nominal masses of type b and type y ions appear above and below the sequence, respectively. Those observed are underlined. Note that the mass difference between y8 and y9, and also b5 and b6, is 243 Da, corresponding to a phosphotyrosine residue.

Arthur R. Salomon, et al. Proc Natl Acad Sci U S A. 2003 January 21;100(2):443-448.
3.
Figure 3

Figure 3. From: Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry.

ZAP-70 Tyr-315,319 phosphorylation in CD3/CD4-stimulated Jurkat cells compared with LIEDNEpYTAR phosphorylation. Total ion chromatogram (TIC) recorded during MS analysis of phosphorylated peptides from unstimulated Jurkat cells (a) and Jurkat cells stimulated with crosslinked anti-CD3 and anti-CD4 antibodies for 1 min (b). Selected-ion chromatogram (SIC) or plot of the ion current vs. time for m/z 990 corresponding to the (M + 3H)3+ of the phosphopeptide ZAP-70 Tyr-315,319 for unstimulated (c) and 1 min stimulated (d) Jurkat cells. SIC for m/z 742.6 corresponding to the (M + 4H)4+ of the phosphopeptide ZAP-70 Tyr-315,319 for unstimulated (e) and 1 min stimulated (f) Jurkat cells. SIC for m/z 686 corresponding to the (M + 2H)2+ of the phosphopeptide LIEDNEpYTAR for unstimulated (g) and 1 min stimulated (h) Jurkat cells.

Arthur R. Salomon, et al. Proc Natl Acad Sci U S A. 2003 January 21;100(2):443-448.

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