Results: 4

1.
FIG. 4.

FIG. 4. From: Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay.

Effect of the immunocapture procedure on the qPCR assay. S. aureus (ATCC 33591; 103 CFU) was mixed with various S. epidermidis titers (MRSE) and analyzed by qPCR following (○) or not following (•) immunocapture enrichment. MRSA in the presence of >100-fold-excess amounts of MRSE was not detected (NA). Values are means ± standard errors of the means for three experiments performed in triplicate.

Patrice Francois, et al. J Clin Microbiol. 2003 January;41(1):254-260.
2.
FIG. 2.

FIG. 2. From: Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay.

Immunocapture of MRSA from mixed cultures. 5 (A), 60 (B), or 1,000 (C) CFU of MRSA (ATCC 33591) was mixed with increasing titers of MRSE, yielding ratios ranging from 1:1 to 1:1,000 for S. aureus (black bars) versus S. epidermidis (grey bars). Immunocapture was evaluated by viable CFU counts performed on MH agar. Means ± ranges for two experiments performed in duplicate are shown. ∗, <10 CFU of MRSE.

Patrice Francois, et al. J Clin Microbiol. 2003 January;41(1):254-260.
3.
FIG. 3.

FIG. 3. From: Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay.

Linearity and limits of detection of the qPCR assay. The linear response of the qPCR mecA assay as a function of input genomic DNA (2 fg to 10 ng) purified from strain ATCC33591 is shown. Correlation coefficient, >0.99; slope, −3.59. Values are means ± standard errors of the means of 4 to 16 determinations. ∗, value excluded from linear regression. ∗∗, amounts of input DNA leading to irregular signal detection.

Patrice Francois, et al. J Clin Microbiol. 2003 January;41(1):254-260.
4.
FIG. 1.

FIG. 1. From: Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay.

Effect of antibody concentration on S. aureus recovery rate. Cowan I strain (NCTC8530; 103 CFU) was used to evaluate the optimal concentration of anti-spa antibodies required for the enrichment step. (A) Recovery was determined by CFU counts of immunocaptured colonies. The curve showed maximal recovery at an antibody dilution of 1:666. (B). Using this optimized anti-spa dilution, recovery was assessed across >7 orders of magnitude in inoculum size. Average values ± standard errors of the means for four experiments performed in duplicate are given.

Patrice Francois, et al. J Clin Microbiol. 2003 January;41(1):254-260.

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