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1.
Fig 2.

Fig 2. From: Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements.

Cluster of orthologous genes examining the MCM N terminus (COG1884) and C terminus (COG2185) and the genes identified as comprising COG1703. Bacteria discussed in this study include Archaeoglobus fulgidus (aful) and Escherichia coli K12 (ecol); others are listed at www.ncbi.nlm.nih.gov/COG.

C. Melissa Dobson, et al. Proc Natl Acad Sci U S A. 2002 November 26;99(24):15554-15559.
2.
Fig 7.

Fig 7. From: Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements.

Mutations reported here and heteroduplex tests. (A) Heteroduplex test for c.592.delACTG: lane 1, control cells; lane 2, WG2014 mixed with control cells in a 1:1 ratio; lane 3, WG2014 alone; lane 4, WG1192 alone; lane 5, WG1192 mixed with control; lane 6, WG1943 mixed with control cells in a 1:1 ratio. (B) Heteroduplex for c.259.delATAAACTT: lane 1, WG3080 alone; lane 2, WG3080 and control cells; lane 3, control cells alone.

C. Melissa Dobson, et al. Proc Natl Acad Sci U S A. 2002 November 26;99(24):15554-15559.
3.
Fig 6.

Fig 6. From: Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements.

Northern blot analysis of MMAA mRNA. A Northern blot of poly(A)+ RNA from the indicated human tissues. The membranes were hybridized with a 32P-labeled 508-bp probe from MMAA mRNA (A) or a β-actin probe (B). Molecular size marker positions are indicated on the right.

C. Melissa Dobson, et al. Proc Natl Acad Sci U S A. 2002 November 26;99(24):15554-15559.
4.
Fig 4.

Fig 4. From: Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements.

Nucleotide and deduced amino acid sequence of human MMAA. The nucleotide and amino acid residues are numbered on the left and right margins, respectively. Two in-frame stop codons are indicated by *** immediately upstream and downstream of the coding sequence. A candidate polyA signal starting at 1,296 is underlined.

C. Melissa Dobson, et al. Proc Natl Acad Sci U S A. 2002 November 26;99(24):15554-15559.
5.
Fig 1.

Fig 1. From: Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements.

Intracellular processing of Cbl (modified from ref. 2). Steps are identified according to complementation group names. Their gene (uppercase italics) and predicted functions are: cblA (MMAA), this study; cblB, cob(I)alamin adenosyltransferase; cblC/D, possibly cob(III)alamin reductase; cblE (MTRR), methionine synthase reductase; cblF, lysosomal Cbl efflux; cblG (MTR), methionine synthase; and cblH, possibly a component of Cbl transport or cob(II)alamin reductase.

C. Melissa Dobson, et al. Proc Natl Acad Sci U S A. 2002 November 26;99(24):15554-15559.
6.
Fig 5.

Fig 5. From: Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements.

Alignment of amino acid sequences similar to that of human (Hum) MMAA, including mouse (Mou), Caenorhabditis elegans (Cel), E. coli (Eco), Mycobacterium tuberculosis (Mtu), and A. fulgidus (Afu). Sequence motifs defining the G3E family of proteins are indicated, including the GxxGxGK[S/T] Walker A, the Mg2+-binding aspartate residue, the DHbHbHbHbE Walker B motif with Hb denoting hydrophobic residues, and an [N/T]KxD GTP-binding motif, as described by Leipe et al. (27). The arrow denotes the predicted mitochondrial leader sequence cleavage site. Amino acids that are similar in all species are shaded with a gray background.

C. Melissa Dobson, et al. Proc Natl Acad Sci U S A. 2002 November 26;99(24):15554-15559.
7.
Fig 3.

Fig 3. From: Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements.

Structural organization of the human MMAA gene. The top schematic (“Genes”) illustrates the flanking genes and map positions surrounding MMAA according to contigview in Ensembl (www.ensembl.org). Note that NCBI maps MMAA to the 147.36–147.38 megabase pairs (Mbp) location of chromosome 4. Both Ensembl and NCBI localize MMAA to 4q31.21–22. The next line (“Exons”) illustrates the location of exons (numbered boxes) and introns (lines) along the MMAA gene located in Contig NT_016606.11. Our independently confirmed sequences from genomic DNA were deposited in GenBank under the accession nos. –. Introns are drawn to scale, except where “?” is used to denote a region of unknown sequence and length and “//” is used to denote a longer region than illustrated. Next the cDNA (“cDNA”) is illustrated, with splice and start/stop codons indicated, showing that it was constructed from the numerous ESTs (“ESTs”) belonging to Unigene clusters Hs.126261 (10 ESTs) and Hs.21017 (18 ESTs) and verified by RT-PCR sequencing. Only some ESTs are illustrated here.

C. Melissa Dobson, et al. Proc Natl Acad Sci U S A. 2002 November 26;99(24):15554-15559.

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