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Results: 6

1.
Fig 6.

Fig 6. From: Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice.

Model for KIF17 function. Schematic of the involvement of motor protein KIF17 in the transport of NMDA receptors and enhancement of learning and memory. The relationship between transport of NR2B by KIF17, synaptic events, involvement of CREB, and transcriptional regulation of NR2B and of KIF17 by phosphorylated CREB.

Richard Wing-Chuen Wong, et al. Proc Natl Acad Sci U S A. 2002 October 29;99(22):14500-14505.
2.
Fig 2.

Fig 2. From: Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice.

Normal performance of GFPKIF17 transgenic mice in open field test. The results show that two groups of mice explore the activity chamber similarly. There were no significant group differences (Tg-1, n = 12; WT, n = 12) in activity indices such as (A) running velocity (P > 0.5 ANOVA) and (B) percentage of time spent on margin region from the wall (P > 0.5 ANOVA), (C) percentage of time spent in central area (P > 0.05 ANOVA), and (D) total distance traveled (P > 0.05 ANOVA) during the 10-min test.

Richard Wing-Chuen Wong, et al. Proc Natl Acad Sci U S A. 2002 October 29;99(22):14500-14505.
3.
Fig 3.

Fig 3. From: Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice.

Enhanced performance of working memory task by GFPKIF17 transgenic mice. (A) Escape latencies in the first five trials of new platform training, averaged from the last two training sessions (Tg-1, n = 12; WT, n = 12) [F (1, 22) = 5.655, P < 0.05 ANOVA]. (B) The time saved (reduction in latencies) between the first and second trials of each session averaged from the last two training sessions (P < 0.05 ANOVA). (C) Swimming velocities averaged from the first two trials of the last two training sessions. No significant difference was observed between the mutant and WT mice.

Richard Wing-Chuen Wong, et al. Proc Natl Acad Sci U S A. 2002 October 29;99(22):14500-14505.
4.
Fig 1.

Fig 1. From: Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice.

Construction and histological characterization of GFPKIF17 transgenic mice. (A) The construct for production of GFPKIF17 transgenic mice. (B) PCR screening with GFP primers, using endogenous MPG as internal control. (C) Visualization of motility of GFPKIF17. (Bar = 20 μm.) (D) Immunoprecipitation of the forebrain fraction from mouse using anti-GFP antibody; the same immunoblot was used for antibodies against KIF17, Mint 1(mLin10), NR2B, and IgG. (E) Normal brain morphology in transgenic mice compared with WT, hematoxylin/eosin staining (Top), higher magnification of the hematoxylin/eosin staining (Middle), and Nissl staining (Bottom) of the hippocampus. (Bars = 500 μm.) (F) The ultrastructure of axospinous synapses in CA1 hippocampus shown in each case. (Bar = 10 μm.) (G) GFPKIF17 localization in forebrain region of mice. Neocortex and hippocampal subregions (CA1, CA3, DG). (Bar = 100 μm.)

Richard Wing-Chuen Wong, et al. Proc Natl Acad Sci U S A. 2002 October 29;99(22):14500-14505.
5.
Fig 5.

Fig 5. From: Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice.

Biochemical and genomic studies of GFPKIF17 mice. (A) Immunoblot analysis of KIF17 protein and NR2B-associated proteins in total forebrain extracts from hippocampus (HP) and cortex (CTX) of 10-wk-old mice of both transgenic lines (Tg-1 and -4) and WT (Left, hippocampus; Right, cortex panels). (B) Expression of NR2B mRNA in GFPKIF17 transgenic mice as determined by RT-PCR analysis. (C) Ratio of NR2B mRNA to GAPDH in GFPKIF17 and WT mice. (D) Immunoblot analysis of the expression level of CREB in forebrain homogenate and nucleus extract of WT and transgenic mice using an antibody specific for unphosphorylated CREB and CREB that is phosphorylated at Ser-133. (E) Ratio of phosphorylated CREB in GFPKIF17 and WT mice forebrain extract. (F) Characterization and nucleotide sequence of the 5′ end of the mouse KIF17 upstream of the 1.0-kb promoter region. (G) Summary of transcription factors present in KIF17 and associated proteins.

Richard Wing-Chuen Wong, et al. Proc Natl Acad Sci U S A. 2002 October 29;99(22):14500-14505.
6.
Fig 4.

Fig 4. From: Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice.

Enhanced performance of spatial learning and memory task by GFPKIF17 transgenic mice. (A) Escape latency in Morris water maze task (Tg-1, n = 12; wild-type, n = 12, mean ± SEM) [F (1, 22) = 8.945, P < 0.01 ANOVA]. The GFPKIF17 mice showed significantly shorter escape latencies than WT mice on the sessions. (B) Location preference in the transfer test conducted at the end of the third training session. GFPKIF17 transgenic mice spent more time in the target quadrant than other quadrants, whereas WT mice did not show any preference for the target quadrant at this stage (P < 0.05, Dunnett's test). (C) Location preference in the second transfer test carried out at the end of the sixth training session. Both transgenic and WT mice exhibited a strong preference for the target quadrant where the hidden platform was previously located. There were no significant differences between the genotypes.

Richard Wing-Chuen Wong, et al. Proc Natl Acad Sci U S A. 2002 October 29;99(22):14500-14505.

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