Results: 3

1.
Figure 3.

Figure 3. From: cdk4 Deficiency Inhibits Skin Tumor Development but Does Not Affect Normal Keratinocyte Proliferation.

Histological analysis of skin lesion 3 days following wounding. Representative paraffin sections of hyperplastic skin from cdk4-deficienct (A) and wild-type (B) epidermis three days after wounding. Hematoxylin and eosin staining. E, epidermis; HE, hyperproliferative epithelium.

Marcelo L. Rodriguez-Puebla, et al. Am J Pathol. 2002 August;161(2):405-411.
2.
Figure 1.

Figure 1. From: cdk4 Deficiency Inhibits Skin Tumor Development but Does Not Affect Normal Keratinocyte Proliferation.

Mouse skin tumor development in cdk4-deficient mice. A: Multiplicity and incidence of papilloma development in cdk4-deficient (KO), cdk4-Heterozygous (Het) and wild-type mice (Wt). Data are expressed as average number of papillomas per mouse (multiplicity) and percentage of mice, which developed papillomas (incidence) as a function of weeks of promotion. B: Representative paraffin-sections of papillomas at 15 weeks from cdk4 KO (−/−) and cdk4 heterozygous (+/−) mice stained with hematoxylin/eosin. cdk4-Null tumors are well-differentiated without foci of dysplasia presenting a histopathology indistinguishable from that of the cdk4+/− and cdk4+/+ (not shown).

Marcelo L. Rodriguez-Puebla, et al. Am J Pathol. 2002 August;161(2):405-411.
3.
Figure 2.

Figure 2. From: cdk4 Deficiency Inhibits Skin Tumor Development but Does Not Affect Normal Keratinocyte Proliferation.

Histological and biochemical analysis of cdk4-null skin. Representative paraffin-sections of skin from wild-type (A, C) and cdk4 KO mice (B, D) were stained with hematoxylin/eosin. A and B, normal skins; C and D, hyperplastic skins induced by topical application of TPA. BrdU incorporation of paraffin sections of hyperplastic skin from wild-type (E) and cdk4-deficient (F) mice was detected with mouse monoclonal anti-BrdU antibody. G: Fresh epidermal protein lysates of cdk4-null (KO) and wild-type sibling (WT) mice were immunoprecipitated with polyclonal anti-cdk4, anti-cdk6, and anti-cdk2 antibodies and an in vitro kinase assay, with pRb as a substrate, was performed. Immunoprecipitation with normal rabbit IgG (NR) was carrying out as negative control. The level of Rb peptide phosphorylation was quantified with a densitometer and the values normalized as percentage of the maximum value. The values are the average of three independent assays.

Marcelo L. Rodriguez-Puebla, et al. Am J Pathol. 2002 August;161(2):405-411.

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