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2.
Figure 4

Figure 4. From: Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases.

Polymerase variants can improve dye–acyNTP terminator incorporation. The incorporation efficiencies of TAMRA–acyCTP by Vent, Vent A488L, Vent Y499L and 9°N A485L DNA polymerases were compared using the titration assay. Numbers refer to the ratio TAMRA–acyCTP:dNTP in the reaction mixture.

Andrew F. Gardner, et al. Nucleic Acids Res. 2002 January 15;30(2):605-613.
3.
Figure 2

Figure 2. From: Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases.

ROX dye attachment enhances both dideoxy and acyclic terminator addition by Vent DNA polymerase. (A) Titration assays were used to compare incorporation of the indicated terminators by Vent DNA polymerase. Numbers refer to the ratio of terminator analog to dNTP. (B) Relative incorporation of ddCTP, acyCTP, ROX–ddCTP and ROX–acyCTP by Vent DNA polymerase is diagrammed to emphasize the elements that enhance terminator incorporation.

Andrew F. Gardner, et al. Nucleic Acids Res. 2002 January 15;30(2):605-613.
4.
Figure 5

Figure 5. From: Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases.

DNA sequence determination with dye–acyNTP terminators. The output of an NEN Model 4200 Global IR2 automated DNA sequencer is illustrated. Reactions were generated with 9°N A485L DNA polymerase. pUC19 was sequenced with primer S1224 using IR700–acyNTPs. Confidence levels for this sequencing reaction are given in Table .

Andrew F. Gardner, et al. Nucleic Acids Res. 2002 January 15;30(2):605-613.
5.
Figure 6

Figure 6. From: Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases.

Incorporation enhancement attributable to terminator and polymerase variations. Incorporation is represented on a logarithmic scale relative to ddCTP incorporation by Vent DNA polymerase. Values for ThermoSequenase incorporation of ddCTP are inferred from Tabor and Richardson (20). Taq DNA polymerase failed to incorporate detectable amounts of acyCTP and thus is not represented. ROX analog incorporation was only examined for Vent and Vent A488L DNA polymerases.

Andrew F. Gardner, et al. Nucleic Acids Res. 2002 January 15;30(2):605-613.
6.
Figure 3

Figure 3. From: Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases.

Hyperthermophilic Family B archaeon DNA polymerases share terminator incorporation properties. Incorporation efficiencies of ROX-acyCTP by Family B DNA polymerases Vent, Deep Vent, Pfu and 9°N DNA polymerases were compared using the titration assay. Numbers refer to the ratio ROX–acyCTP:dNTP in the reaction mixture.

Andrew F. Gardner, et al. Nucleic Acids Res. 2002 January 15;30(2):605-613.
7.

Figure 1. From: Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases.

Incorporation of ddCTP and acyCTP. Titration assay for the indicated terminators using (A) Taq (B) Vent, (C) ThermoSequenase or (D) Vent A488L DNA polymerase. Extension of a 32P-labeled primer on a M13mp18 single-stranded substrate was examined in the presence of the indicated ratios of terminator to dNTP. The lane marked dNTP is a control reaction performed in the absence of terminators.

Andrew F. Gardner, et al. Nucleic Acids Res. 2002 January 15;30(2):605-613.

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