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Results: 4

1.

Figure. From: A role for RAD54B in homologous recombination in human cells.

Fig. 2. Gene targeting at the human XRCC2 locus. (A) Schematic representation of the XRCC2 locus, the targeting vector and the targeted locus. Relevant XhoI (Xh), SacI (S), HincII (Hc) and XbaI (Xb) restriction sites, and the position of the probe used for Southern blot analysis are shown. The positions of the primers used for PCR are indicated by arrows. Exon III is indicated by a numbered solid box. (B) Southern blot analysis of DNA from HCT116 cells digested with HincII or XbaI. Both blots were hybridized with the probe indicated in (A).

Kiyoshi Miyagawa, et al. EMBO J. 2002 January 15;21(1-2):175-180.
2.

Figure. From: A role for RAD54B in homologous recombination in human cells.

Fig. 3. Gene targeting at the human RAD51L2/RAD51C locus. (A) Schematic representation of the RAD51L2/RAD51C locus, the targeting vector and the targeted locus. Relevant XhoI (Xh), XbaI (Xb), DraIII (D), BamHI (B) and HindIII (H) restriction sites, and the position of the probe used for Southern blot analysis are shown. The positions of the primers used for PCR are indicated by arrows. Exons I, II and III are indicated by numbered solid boxes. (B) Southern blot analysis of DNA from HCT116 cells digested with BamHI or HindIII. Both blots were hybridized with the probe indicated in (A).

Kiyoshi Miyagawa, et al. EMBO J. 2002 January 15;21(1-2):175-180.
3.

Figure. From: A role for RAD54B in homologous recombination in human cells.

Fig. 1. Gene targeting at the human RAD54B locus. (A) Schematic representation of the RAD54B locus, the targeting vectors and the targeted locus. Relevant EcoRI (E), SacI (S) and BglII (Bg) restriction sites, and the position of the probe used for Southern blot analysis are shown. Exon II is indicated by a numbered solid box. (B) Southern blot analysis of DNA from HCT116 cells digested with SacI or BglII. Both blots were hybridized with the probe indicated in (A). (C) Western blot analysis of whole-cell extracts from HCT116 cells. The blot was incubated with a 1:200 dilution of anti-Rad54B antiserum.

Kiyoshi Miyagawa, et al. EMBO J. 2002 January 15;21(1-2):175-180.
4.

Figure. From: A role for RAD54B in homologous recombination in human cells.

Fig. 4. Proliferative characteristics, sensitivity to DNA-damaging agents and levels of SCE of wild-type and targeted HCT116 cells. (A) Growth curves. (B) Sensitivity to ionizing radiation. (C) Sensitivity to MMS. (D) Sensitivity to cisplatin. All measurements in growth curves and cell survival were performed in triplicate. Results of representative experiments are shown here. Consistent results were obtained between different sets of experiments. (E) Levels of SCE. The RAD54B-deficient cell line used in this study was RAD54B//b125. One hundred cells were analyzed in each preparation. The mean number of SCEs per metaphase and the standard deviation are shown.

Kiyoshi Miyagawa, et al. EMBO J. 2002 January 15;21(1-2):175-180.

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