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Results: 4

1.
Figure 1.

Figure 1. From: Features of Colorectal Cancers with High-Level Microsatellite Instability Occurring in Familial and Sporadic Settings .

Examples of microsatellite instability at D17S250, BAT26, D5S346, and MYCL in groups of normal mucosa/tumor (N/T) pairs. Asterisks indicate a positive result.

Joanne Young, et al. Am J Pathol. 2001 December;159(6):2107-2116.
2.
Figure 2.

Figure 2. From: Features of Colorectal Cancers with High-Level Microsatellite Instability Occurring in Familial and Sporadic Settings .

Immunohistochemical demonstration of MMR gene deficiency. Brown color indicates the presence of the protein under study. 1 shows the concomitant loss of MLH1 and PMS2, and 2 shows the concomitant loss of MSH2 and MSH6 in the poorly differentiated cancer on the right of the respective figures. Note positive staining in stromal cells and normal mucosa in sections where MMR expression is lost.

Joanne Young, et al. Am J Pathol. 2001 December;159(6):2107-2116.
3.
Figure 3.

Figure 3. From: Features of Colorectal Cancers with High-Level Microsatellite Instability Occurring in Familial and Sporadic Settings .

hMLH1 methylation studies on two normal mucosa/tumor (N/T) pairs. In both instances the normal mucosa/tumor pair on the left is from a methylated cancer and that on the right from an unmethylated cancer. a: The results of methylation-specific PCR. NU, normal mucosa DNA amplified with a methylation-insensitive primer; TM, cancer DNA amplified with a methylation-sensitive primer. Note that the unmethylated cancer is not detected using the methylation-sensitive primer. b: The COBRA assay. N, normal mucosa DNA; T, cancer DNA. Note that the PCR product has been cleaved into restriction fragments in the methylated cancer.

Joanne Young, et al. Am J Pathol. 2001 December;159(6):2107-2116.
4.
Figure 4.

Figure 4. From: Features of Colorectal Cancers with High-Level Microsatellite Instability Occurring in Familial and Sporadic Settings .

A: Poorly differentiated sporadic MSI-H adenocarcinoma showing epithelium arranged in irregular clusters and trabeculae. B: Medullary type and lymphocyte-rich carcinoma from subject with HNPCC. C: Poorly differentiated adenocarcinoma from subject with HNPCC carcinoma composed of epithelium arranged in islands. D: Well-differentiated mucinous carcinoma in which epithelium is lined by tall goblet cells and from a subject with HNPCC. E: Moderately differentiated adenocarcinoma from subject with HNPCC showing tumor-infiltrating (intraepithelial) lymphocytes. F: Residual serrated adenoma adjacent to a sporadic MSI-H colorectal cancer (not shown). G: Example of two distinct subclones, one poorly differentiated medullary type and the second mucinous from a sporadic MSI-H cancer. H: Sporadic MSI-H colorectal cancer formed of well-differentiated papillae covered by a serrated epithelium and associated with moderate amounts of extracellular mucin. I: Sporadic MSI-H colorectal cancer showing retention of serrated crypt morphology in association with abundant extracellular mucin. H&E; original magnifications: ×80 (A, F, G); ×160 (B, C, D); ×320 (H and I); and ×800 (E).

Joanne Young, et al. Am J Pathol. 2001 December;159(6):2107-2116.

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