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Results: 4

1.
FIG. 4

FIG. 4. From: Killing Effect and Antitoxic Activity of the Leptospira interrogans Toxin-Antitoxin System in Escherichia coli.

Growth of E. coli expressing L. interrogans ChpK in the presence or absence of 100 μM IPTG. Cells harboring pET30a, pTAK (chpK), and pTAKI (chpK chpL) are indicated. Plates supplemented with 50 μg of kanamycin (Km) per ml were incubated for 24 h at 37°C.

Mathieu Picardeau, et al. J Bacteriol. 2001 November;183(21):6494-6497.
2.
FIG. 3

FIG. 3. From: Killing Effect and Antitoxic Activity of the Leptospira interrogans Toxin-Antitoxin System in Escherichia coli.

Effect of the expression of the L. interrogans ChpK in the presence or absence of ChpI on growth of E. coli cells harboring pET30a (filled square), pTAK (chpK with an AUG start codon) (open circles), pTGK (chpK with a GUG start codon) (open squares), and pTAKI (chpK and chpI) (open triangles) in LB liquid media. Growth was determined by measuring the optical density at 600 nm.

Mathieu Picardeau, et al. J Bacteriol. 2001 November;183(21):6494-6497.
3.
FIG. 1

FIG. 1. From: Killing Effect and Antitoxic Activity of the Leptospira interrogans Toxin-Antitoxin System in Escherichia coli.

Genetic structure of the chp locus of L. interrogans. (A) Schematic representation of the chp locus. The arrows for chpI and chpK show their transcription orientation. IRs are boxed. (B) Sequence alignment of the putative ChpK proteins of L. interrogans (Lint), M. tuberculosis (Mtub), B. subtilis (Bsub), and S. aureus (Saur) and of the PemK protein of E. coli (Ecol). Residues conserved in at least three homologous proteins are shaded.

Mathieu Picardeau, et al. J Bacteriol. 2001 November;183(21):6494-6497.
4.
FIG. 2

FIG. 2. From: Killing Effect and Antitoxic Activity of the Leptospira interrogans Toxin-Antitoxin System in Escherichia coli.

Southern blot analyses of Leptospira spp. Southern blotting of EcoRI-digested DNA was performed as previously described (8), and blots were probed at 50°C with the radiolabeled chpK probe from L. interrogans serovar icterohaemorrhagiae strain Lai. The chpK probe was amplified by PCR with primers PkA (5′-TTC ATT TGG AAG TGA GCC TG-3′) and PkB (5′-AAT CTA AGC CTG TAA CCA AC-3′). Lane 1, L. interrogans serovar icterohaemorrhagiae strain Lai; lane 2, L. interrogans serovar copenhageni strain Wijnberg; lane 3, L. interrogans serovar icterohaemorrhagiae strain Verdun; lane 4, L. interrogans serovar grippotyphosa strain Moskva V; lane 5, L. interrogans serovar canicola strain Hond Utrecht IV; lane 6, L. interrogans serovar sejroe strain M84; lane 7, L. biflexa serovar patoc strain Patoc1; lane 8, L. meyeri serovar semaranga strain Veldrat.

Mathieu Picardeau, et al. J Bacteriol. 2001 November;183(21):6494-6497.

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