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Results: 5

1.
Figure 4.

Figure 4. From: Sodium channel ?1 and ?3 subunits associate with neurofascin through their extracellular immunoglobulin-like domain.

The extracellular domain of β1 interacts with neurofascin. (A) TsA-201 cells cotransfected with NF186Δic and β1 were lysed and probed with the indicated antibodies. Blots were probed with anti-HA.11. Cell lysates were also probed with anti-HA.11 and anti-β1CT to show that both proteins were expressed. (B) Cells cotransfected with neurofascin and β1β2β2 were lysed and immunoprecipitated (IP) with the indicated antibody. Blots were probed with anti-HA.11. Cell lysates were probed with anti-HA.11 and anti-β2CT to show expression of both proteins. (C) Cells cotransfected with β1ec–GPI and neurofascin were lysed and immunoprecipitated with antibodies as indicated. Cell lysates were probed with anti-HA.11 or anti-β1extra to show both proteins were expressed.

Charlotte F. Ratcliffe, et al. J Cell Biol. 2001 July 23;154(2):427-434.
2.
Figure 1.

Figure 1. From: Sodium channel ?1 and ?3 subunits associate with neurofascin through their extracellular immunoglobulin-like domain.

Sodium channel β1 and β3 subunits coimmunoprecipitate with neurofascin in cotransfected tsA-201 cells. (A) Representation of HA.11-tagged neurofascin 186. (B) TsA-201 cells were cotransfected with neurofascin 186 (NF186) and sodium channel subunits as indicated. Cell lysates were immunoprecipitated with the indicated antibodies and the blot was probed with monoclonal antibody anti-HA.11. Each cell lysate was probed with anti-HA.11 or specific antisodium channel antibodies as shown to confirm that all proteins were expressed (bottom).

Charlotte F. Ratcliffe, et al. J Cell Biol. 2001 July 23;154(2):427-434.
3.
Figure 3.

Figure 3. From: Sodium channel ?1 and ?3 subunits associate with neurofascin through their extracellular immunoglobulin-like domain.

β1 and neurofascin associate in cis but not in trans. (A) TsA-201 cells cotransfected with β1 and neurofascin (NF) were lysed and immunoprecipitated (IP) as described previously. (B) TsA-201 cells were transfected separately and cocultured. Lysed cells were immunoprecipitated with the indicated antibodies and the blot was probed with anti-HA.11. The bottom panel shows cell lysates probed with anti-β1CT or anti-HA.11 to demonstrate that proteins were well expressed. (C) Cells transfected with β1 were stained with anti-β1CT, and (D) cells transfected with HA-tagged neurofascin 186 were stained with anti-HA.11.

Charlotte F. Ratcliffe, et al. J Cell Biol. 2001 July 23;154(2):427-434.
4.
Figure 2.

Figure 2. From: Sodium channel ?1 and ?3 subunits associate with neurofascin through their extracellular immunoglobulin-like domain.

Neurofascin and β1 localize to nodes of Ranvier and associate in rat brain. Rat sciatic nerve was labeled with neurofascin antibody anti-FNIII (A) or anti-β1CT (B). Expression of both β1 and neurofascin is concentrated at nodes of Ranvier. (C) P5 or adult rat brain lysates were immunoprecipitated with the indicated antibodies. Blots were probed with neurofascin antimucin antibody. (D) Expression of β1 in both P5 and adult rat brain lysates. (E and F) Teased sciatic nerve stained with anti-β1extra from 3-d-old (E) and 10-d-old (F) rats, demonstrating the localization of β1 at the nodes of Ranvier in development. NF, neurofascin. Bars: 2 μm (A and B) and 10 μm (E and F).

Charlotte F. Ratcliffe, et al. J Cell Biol. 2001 July 23;154(2):427-434.
5.
Figure 5.

Figure 5. From: Sodium channel ?1 and ?3 subunits associate with neurofascin through their extracellular immunoglobulin-like domain.

Determination of the neurofascin binding site. (A) TsA-201 cells were cotransfected with α and β1 subunits and GPI-tagged constructs Ig1-GPI, Ig2-GPI, Ig3-GPI, Ig4-GPI, Ig5-GPI, Ig6-GPI, FN1-GPI, FN2-GPI, FN4-GPI, and mucin-GPI, respectively. Cell lysates were immunoprecipitated with anti-SP20 and blots were probed with anti-HA.11 antibody. (B) Lysates were probed with anti-SP20, anti-β1CT, and anti-HA.11 to demonstrate that all proteins were expressed. (C) TsA-201 cells were cotransfected with (lane 1) α, β1, and neurofascin 186; (lane 2) α and neurofascin 186; (lane 3) α, β1, and Ig1-GPI; (lane 4) α and Ig1-GPI; (lane 5) α, β1, and FN2-GPI; and (lane 6) α and FN2-GPI. Cell lysates were immunoprecipitated with anti-SP20 and blots were probed with anti-HA.11 antibody.

Charlotte F. Ratcliffe, et al. J Cell Biol. 2001 July 23;154(2):427-434.

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