Results: 5

1.
Figure 3

Figure 3. From: Trace amines: Identification of a family of mammalian G protein-coupled receptors.

β-PEA-induced responses in COS-7 cells transfected with human TA1, rat TA2, or vector. Cells were incubated with increasing concentrations of β-PEA and cAMP accumulation measured. Data are from duplicate determinations and are representative of three to six experiments.

Beth Borowsky, et al. Proc Natl Acad Sci U S A. 2001 July 31;98(16):8966-8971.
2.
Figure 2

Figure 2. From: Trace amines: Identification of a family of mammalian G protein-coupled receptors.

Voltage-clamp responses to trace amines in oocytes. (A) Response to 100 μM octopamine (Oct) or 5-HT in an oocyte expressing rat TA1 and CFTR. (B) Response to 100 nM tyramine (Tyr) in an oocyte expressing rat TA1 and CFTR. (C) Response to 100 μM octopamine in an oocyte expressing only TA1. (D) Response to 100 nM tyramine (Tyr) in an oocyte expressing human TA1 and CFTR. Holding potential was −80 mV for all oocytes. Marker bar in D also applies to A and C.

Beth Borowsky, et al. Proc Natl Acad Sci U S A. 2001 July 31;98(16):8966-8971.
3.
Figure 1

Figure 1. From: Trace amines: Identification of a family of mammalian G protein-coupled receptors.

Alignment of rat, mouse, and human TA1 and rat TA2 receptors (GenBank accession nos. AF380186, AF380187, AF380185, and AF380188, respectively). Shaded residues are conserved in all four receptors. Triangles and circles indicate residues conserved in TA1–TA15. Open triangles are also conserved among all human monaminergic receptors, and open circles are conserved among all human 5-HT but not NE or DA receptors. Seven putative TM domains are indicated.

Beth Borowsky, et al. Proc Natl Acad Sci U S A. 2001 July 31;98(16):8966-8971.
4.
Figure 4

Figure 4. From: Trace amines: Identification of a family of mammalian G protein-coupled receptors.

A phylogenetic tree for trace amine receptors TA1–TA15, human 5-HT receptors 5-HT1a, 5-HT1b, 5-HT1d, 5-HT4, 5-HT5, 5-HT7, human α1a receptor (AR-α1a), GPR57, GPR58, PNR, 5-HT4 psuedogene (5-HT), drosophila (Drosoph) receptors for octopamine (Oct), 5-HT1 and tyramine (Tyr), Caenorhabditis elegans (C. Elegans) 5-HT receptor, tyramine receptors from bee (Bee Tyr) and locust (Locust Tyr), and a snail octopamine receptor (Snail Oct). Amino acid sequences for each receptor spanning from the start of TMI to the end of TMVII were aligned by using the CLUSTALW algorithm and the tree constructed by the NJ method on a DecypherII Bioaccelerator (TimeLogic, Reno, NV). GenBank accession nos.: AF380190 (rat TA3), AF380189 (human TA3), AF380191 (rat TA4), AF380192 (human TA4), AF380193-AF380203 (TA5–TA15).

Beth Borowsky, et al. Proc Natl Acad Sci U S A. 2001 July 31;98(16):8966-8971.
5.
Figure 5

Figure 5. From: Trace amines: Identification of a family of mammalian G protein-coupled receptors.

Photomicrographs showing hybridization signals for TA1 mRNA in mouse CNS. Signal detected in cerebellar Purkinje cells (arrows) hybridized with antisense (A and B) and sense (C) probes. Scale bar in C (200 μm) also applies to A and E; scale bar in B, 25 μm. Photomicrographs showing hybridization signal in cells (arrows) in the dorsal raphe (D), ventral tegmental area (VTA), substantia nigra, compact part (SNc) and reticular part (SNr) (E), and locus coeruleus (LC; F). Scale bar in F (50 μm) also applies to D. MPB, medial parabrachial nucleus; Me5, mesencephalic trigeminal nucleus; m, medial longitudinal fasciculus; scp, superior cerebral peduncle.

Beth Borowsky, et al. Proc Natl Acad Sci U S A. 2001 July 31;98(16):8966-8971.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk