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Results: 5

1.
Figure 1

Figure 1. From: In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore.

The generic chemical structure of bilin chromophores. The conventional numbering of carbon atoms on the linear tetrapyrrole backbone and lettering of the four pyrrole rings are shown on the structure. R1–R9 represent side chains of the synthesized bilins.

Hiroko Hanzawa, et al. Proc Natl Acad Sci U S A. 2001 March 13;98(6):3612-3617.
2.
Figure 4

Figure 4. From: In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore.

Difference absorption spectra of the PHYB adduct with PCB analogs having side chain substituents in D-ring (analogs 13–20; see Table 1). The difference spectra were obtained as described in the legend to Fig. 2. Black and gray dotted lines indicate the difference spectra obtained after the first and second cycles, respectively. The vertical bars represent 0.002 absorbance difference units.

Hiroko Hanzawa, et al. Proc Natl Acad Sci U S A. 2001 March 13;98(6):3612-3617.
3.
Figure 3

Figure 3. From: In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore.

Difference absorption spectra of the PHYB adducts with PCB analogs having side-chain substituents in the B- and C-rings (analogs 5–12; see Table 1). The difference spectra were obtained as described in the legend to Fig. 2. Black and gray dotted lines indicate the difference spectra obtained after the first and second cycles, respectively. The vertical bars represent 0.002 absorbance difference units.

Hiroko Hanzawa, et al. Proc Natl Acad Sci U S A. 2001 March 13;98(6):3612-3617.
4.
Figure 2

Figure 2. From: In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore.

Difference absorption spectra of the PHYB adducts with PCB, PΦB, and analogs having side-chain substituents in the A-ring (analogs 1–4; see Table 1). The difference spectra were obtained by subtracting the absorption spectra determined after far-red light irradiation from that measured after red light irradiation. The sample volumes were 800 μl. Difference spectra were recorded after two consecutive red/far-red cycles. Black and gray dotted lines indicate the difference spectra obtained after the first and second cycles, respectively. The vertical bars represent 0.002 absorbance difference units.

Hiroko Hanzawa, et al. Proc Natl Acad Sci U S A. 2001 March 13;98(6):3612-3617.
5.
Figure 5

Figure 5. From: In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore.

Absorption and difference spectra of PhyB assembled with PCB and analog 18 when red and far-red light irradiated. The absorption spectra were determined after actinic red and far-red light irradiation. The difference spectra were obtained as described in the legend to Fig. 2. The sample volumes were 250 μl. Total protein concentrations were 0.82 mg/ml for the PCB adduct fraction and 0.37 mg/ml for the analog 18 adduct. Absorption spectra of adducts with PCB (a) and analog 18 (b) are shown (Upper). Black lines represent spectra measured after far-red light irradiation. Gray lines represent spectra after red light irradiation. Far-red/red difference spectra of adducts with PCB (c) and analog 18 (d) are shown (Lower).

Hiroko Hanzawa, et al. Proc Natl Acad Sci U S A. 2001 March 13;98(6):3612-3617.

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