Results: 3

1.
Figure 1

Figure 1. From: Development of a sensitive multi-well colorimetric assay for active NFκB.

Schematic procedure of the microwell NFκB-DNA binding assay.

Patricia Renard, et al. Nucleic Acids Res. 2001 February 15;29(4):e21-e21.
2.
Figure 2

Figure 2. From: Development of a sensitive multi-well colorimetric assay for active NFκB.

Comparison of the NFκB DNA-binding assay in microwells (A) and by EMSA (B). (A) Microwells containing the DNA probe were incubated with increasing amounts of cell lysates (0.5, 1, 2, 5, 10, 25 and 50 µg of proteins) prepared either from unstimulated cells (open columns) or from IL-1-stimulated cells (black columns). The data represent the means of three values ± SD. (B) EMSA was performed with 0.5, 1, 5, 10, 25 and 50 µg of proteins extracted from unstimulated cells or from IL-1-stimulated cells [same protein extracts as in (A)]. The gel was exposed for 5 days.

Patricia Renard, et al. Nucleic Acids Res. 2001 February 15;29(4):e21-e21.
3.
Figure 3

Figure 3. From: Development of a sensitive multi-well colorimetric assay for active NFκB.

Competition assay, using a wild-type probe and a mutated probe, on the DNA-binding of NFκB, estimated by the microwell assay (A) and by EMSA (B). (A) Microwells bearing the 122 bp DNA probe were incubated with an increasing excess of the 22 bp non-biotinylated probe, containing either the wild-type or the mutated NFκB-binding consensus sequence. The DNA-binding assay was then performed with 5 µg protein of cell lysates prepared either from unstimulated cells (open columns) or from IL-1-stimulated cells (black columns). The data represent the means of three values ± SD. (B) The radioactive 22 bp probe was first mixed with an increasing excess (0, 1, 5 and 20 times) of the cold 22 bp probe containing either the wild-type (lanes 1–4 and 9–12) or the mutated (lanes 4–8 and 13–16) NFκB-binding consensus sequence. EMSA was then performed with 25 µg protein of cell extracts coming from unstimulated (lanes 1–8) or IL-1-stimulated cells (lanes 9–16).

Patricia Renard, et al. Nucleic Acids Res. 2001 February 15;29(4):e21-e21.

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