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Results: 5

1.
Figure 5

Figure 5. From: Immunization against Alzheimer's ?-amyloid plaques via EFRH phage administration.

Interference of immunized sera with fibrillar β-amyloid formation. Fluorescence of ThT correlates with the amount of fibrillar β-amyloid formed after reincubation of Aβ fibril with sera from immunized animal. As control, serum of unimmunized animal was added in the same experimental conditions.

Dan Frenkel, et al. Proc Natl Acad Sci U S A. 2000 October 10;97(21):11455-11459.
2.
Figure 1

Figure 1. From: Immunization against Alzheimer's ?-amyloid plaques via EFRH phage administration.

Binding of the anti-aggregating mAb to ERFH-phage. Antibodies were added to the phage-coated wells, and binding was analyzed by ELISA, as described. ▵, KEPRHHIQHHERVIR (F8-II); ■, VHEPHEFRHVALNPV (C3-II); +, DTEFRHSSNNFSAVR (D7-I); ●, STEFRHQTTPLHPNS (C11-I); ○, SAADFRHGSPPISAF (D3-I); □, wild filamentous phage used as control.

Dan Frenkel, et al. Proc Natl Acad Sci U S A. 2000 October 10;97(21):11455-11459.
3.
Figure 3

Figure 3. From: Immunization against Alzheimer's ?-amyloid plaques via EFRH phage administration.

Long-lasting immunization with f3-EFRH filamentous phage. Serum IgG titer of different bleeds from mice immunized against phage coat proteins and the EFRH epitope on the N terminus of AβP. ■, sera against AβP 1–16; sera against wild filamentous phage.

Dan Frenkel, et al. Proc Natl Acad Sci U S A. 2000 October 10;97(21):11455-11459.
4.
Figure 4

Figure 4. From: Immunization against Alzheimer's ?-amyloid plaques via EFRH phage administration.

Prevention of AβP-mediated toxic effect on PC12 cells by sera against f88-EFRH phage. Cells were incubated with fibrillar Aβ alone, or with fibrillar Aβ that had been incubated with serum from the third bleeding at different concentrations. Serum of unimmunized animal was used as negative control. The MTT assay was used to estimate cell survival.

Dan Frenkel, et al. Proc Natl Acad Sci U S A. 2000 October 10;97(21):11455-11459.
5.
Figure 2

Figure 2. From: Immunization against Alzheimer's ?-amyloid plaques via EFRH phage administration.

Immunization with f88 or f3 filamentous phage displaying EFRH epitope. (A) Diagram of immunization schedule. (B) Serum IgG titer of different bleeds from mice immunized with f3-EFRH or f88-EFRH phage against wild phage coat proteins and the N terminus of β-amyloid peptide (1–16 amino acid) after three i.p. administrations or (C) after single i.n. administration. (D) Serum IgG titer of guinea pigs from animals s.c. immunized with EFRH phage was measured after the third and the fourth administrations.

Dan Frenkel, et al. Proc Natl Acad Sci U S A. 2000 October 10;97(21):11455-11459.

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