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Results: 4

1.
FIG. 2

FIG. 2. From: The LE1 Bacteriophage Replicates as a Plasmid within Leptospira biflexa: Construction of an L. biflexa-Escherichia coli Shuttle Vector.

Subcloning of the LE1 origin of replication. Subcloning of plasmid pGKLep1 was performed to determine the minimum region required for replication in L. biflexa. The locations of some restriction sites used for subcloning are indicated. The ability (+) or the inability (−) of a given clone to replicate in L. biflexa is shown on the right.

Isabelle Saint Girons, et al. J Bacteriol. 2000 October;182(20):5700-5705.
2.
FIG. 1

FIG. 1. From: The LE1 Bacteriophage Replicates as a Plasmid within Leptospira biflexa: Construction of an L. biflexa-Escherichia coli Shuttle Vector.

Evidence for LE1 behaving like a plasmid. DNAs from the L. biflexa control strain Patoc 1 and lysogens 3c and 6c (lanes 1, 2, and 3, respectively) were not digested, restricted with NotI or SgrAI, and blotted on a membrane. [α-P33]dATP-labeled LE1 DNA was used as a probe.

Isabelle Saint Girons, et al. J Bacteriol. 2000 October;182(20):5700-5705.
3.
FIG. 4

FIG. 4. From: The LE1 Bacteriophage Replicates as a Plasmid within Leptospira biflexa: Construction of an L. biflexa-Escherichia coli Shuttle Vector.

Nucleotide sequence of the LE1 origin of replication. (A) Genetic organization of the replication region of pGKLep1. The putative genes and their transcription orientations are indicated by open arrows. Similarities with proteins in databases (accession numbers in brackets) are indicated. (B) Nucleotide sequence of the replication region of pGKLep4. Bold letters indicate repeats and runs of A and T (underlined). Arrows indicate inverted repeats (IR) and direct repeats (DR).

Isabelle Saint Girons, et al. J Bacteriol. 2000 October;182(20):5700-5705.
4.
FIG. 3

FIG. 3. From: The LE1 Bacteriophage Replicates as a Plasmid within Leptospira biflexa: Construction of an L. biflexa-Escherichia coli Shuttle Vector.

Evidence for the autonomous replication of pGKLep1 and pGKLep4 within L. biflexa. Shown are the results of a Southern blot analysis of DNA of L. biflexa transformed with pGKLep1 (lane 2) and pGKLep4 (lane 3). Lanes 1 and 4, original pGKLep1 and pGKLep4 plasmids, respectively. DNAs in all lanes were digested with EcoRI and probed with pGKLep1. Sizes of the fragments of the molecular mass markers are indicated on the left.

Isabelle Saint Girons, et al. J Bacteriol. 2000 October;182(20):5700-5705.

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