Results: 5

1.
FIG. 1

FIG. 1. From: Simian Immunodeficiency Virus Rapidly Penetrates the Cervicovaginal Mucosa after Intravaginal Inoculation and Infects Intraepithelial Dendritic Cells.

Procedure for processing fresh genital tract tissues from animals at necropsy. Note that approximately 90% of the vaginal mucosa was used to produce cell suspensions, while 10% of the tissue was processed for histology or quick-frozen for PCR analysis. Abbreviations: IHC, immunohistochemistry; ISH/IHC, combination of ISH and IHC; IFA, immunofluorescent antibody labeling.

Jinjie Hu, et al. J Virol. 2000 July;74(13):6087-6095.
2.
FIG. 2

FIG. 2. From: Simian Immunodeficiency Virus Rapidly Penetrates the Cervicovaginal Mucosa after Intravaginal Inoculation and Infects Intraepithelial Dendritic Cells.

SIV-infected cells detected by ISH in tissues of rhesus macaques 18 h after vaginal SIV inoculation. (A) SIV RNA+ cells (arrows) in the vaginal mucosa (animal 24659, 18 h PI). Note that one cell is in the middle layer of the epithelium and the other is in the lamina propria. (B) SIV RNA+ cell in the subcapsular sinus of the iliac lymph node, which drains the vagina (animal 24659, 18 h PI). Two double-headed arrows denote the subcapsular sinus of the lymph node. ISH was done with NBT-BCIP as the chromogen and nuclear fast red counterstain.

Jinjie Hu, et al. J Virol. 2000 July;74(13):6087-6095.
3.
FIG. 4

FIG. 4. From: Simian Immunodeficiency Virus Rapidly Penetrates the Cervicovaginal Mucosa after Intravaginal Inoculation and Infects Intraepithelial Dendritic Cells.

Immunophenotypic characterization of SIV RNA+ cells in HLA-ECS cytospin slides of vaginal epithelium at 18 h PI. Panels A to C show A single, high-magnification field in a cytospin slide from animal 23319 (18 h PI). (A) Viewed through an appropriate band-pass filter, SIV RNA+ cells were detected (green, arrows), and some of these cells had dendritic processes (arrowhead). (B) Most cells express p55+ (red), a marker for DC. (C) Viewed through a double band-pass filter, all the SIV RNA+ cells in this field are p55+ DC (arrows). Panels D to F show a single, high-magnification field in a cytospin slide of vaginal epithelium from animal 23319 (18 h PI). (D) SIV RNA+ cells (green, arrows). (E) Most cells (red) express CD1a, a marker for LC. (F) Viewed through a double band-pass filter, all the SIV RNA+ cells in this field are CD1a+ LC (arrows). Combined ISH (digoxigenin-labeled riboprobe, Tyramide-FITC detection system) and immunofluorescent antibody labeling of cell markers (Texas red detection system) were used.

Jinjie Hu, et al. J Virol. 2000 July;74(13):6087-6095.
4.
FIG. 3

FIG. 3. From: Simian Immunodeficiency Virus Rapidly Penetrates the Cervicovaginal Mucosa after Intravaginal Inoculation and Infects Intraepithelial Dendritic Cells.

SIV-infected cells in tissues of rhesus macaques as detected by combined ISH and immunohistochemistry. SIV RNA+ cells in formalin-fixed sections of vagina at 24 h PI are shown. (A) SIV RNA+ cells (arrows) in the vaginal epithelium and uninfected CD3+ T cells (red, some are denoted by arrowheads) in the vaginal epithelium and lamina propria (animal 24294, 24 h PI). (B) Higher-magnification view of panel A. Note that the SIV RNA+ cells are not CD3+ T cells. The solid black line demarcates the basal lamina. (C) SIV RNA+ cell (arrow) in the basal layer of the vaginal epithelium and uninfected HAM 56+ macrophages (red, some are denoted by arrowheads) in the vaginal epithelium and lamina propria (animal 24294, 24 h PI). (D) Higher-magnification view of panel C. Note that the SIV RNA+ cell is not a macrophage and the macrophages are not SIV RNA+ cells. The solid black line demarcates the basal lamina. (E) SIV RNA+ macrophage (arrow) in the paracortex of an iliac lymph node (animal 24294, 24 h PI). Note in panels A and C that the vaginal epithelium is intact at 24 h PI, consistent with the atraumatic virus inoculation procedure. ISH using 35S-labeled SIV riboprobes combined with immunohistochemistry using AEC as the chromogen and Meyer's hematoxylin counterstain were used.

Jinjie Hu, et al. J Virol. 2000 July;74(13):6087-6095.
5.
FIG. 5

FIG. 5. From: Simian Immunodeficiency Virus Rapidly Penetrates the Cervicovaginal Mucosa after Intravaginal Inoculation and Infects Intraepithelial Dendritic Cells.

Overview of the experimental design used to determine the minimum amount of contact time required for SIV to pass from the vaginal lumen into the vaginal mucosa. (A) In experiment A, the initial study, animals were exposed intravaginally to SIV for 15, 30, or 60 min and then the vagina was flushed with vinegar. (B) In experiment B, the animals that did not become infected in experiment A were reexposed to two SIV inoculations and vinegar lavage procedures in a single day with a 4-h resting interval between inoculations. The inoculum was completely inactivated by the vinegar lavage after the first exposure, so the two exposures in a single day should be considered independent transmission opportunities. (C) In experiment C, control animals were lavaged with vinegar, allowed to rest for 4 h, and then exposed intravaginally to SIV for 15 min. (D) In experiment D, control animals were exposed to SIV once. The inoculum was left undisturbed after deposition in the vagina. (D) In experiment E, control animals were exposed to SIV once, allowed to rest for 4 h, and then exposed intravaginally to SIV again. The inoculum was left undisturbed after deposition in the vagina. To assess the results of the experiment, the animals were monitored for 16 weeks for systemic SIV infection and the results are shown in Table 4.

Jinjie Hu, et al. J Virol. 2000 July;74(13):6087-6095.

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