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1.
Figure 2

Figure 2. From: Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis.

Golgin-160 is cleaved during apoptosis. HeLa cells were incubated for 2 h with 1 μm staurosporine. Lysates from control and treated cells were run on a 7.5% SDS-PAGE and immunoblotted with rabbit anti–golgin-160 antiserum. 70 μg of lysate was loaded in each lane. Results are representative of three separate experiments.

Marie Mancini, et al. J Cell Biol. 2000 May 1;149(3):603-612.
2.
Figure 7

Figure 7. From: Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis.

The NH2-terminal fragment of golgin-160 is released from Golgi membranes after cleavage by caspase-2. Untreated HeLa cells (a–d) and HeLa cells treated with 1 μm staurosporine for 1 h (e–h) were fixed, permeabilized, and triple labeled with anti–golgin-160(N) (a and e), which was visualized as red; antigiantin (b and f), which was visualized as green; and DAPI (c and g), which was visualized as blue. Overlapping red and green pixels appear yellow (d and h). Arrow in h denotes an apoptotic cell, which no longer stains for the NH2-terminal portion of golgin-160. These results are representative of three separate experiments. Bars, 10 μm.

Marie Mancini, et al. J Cell Biol. 2000 May 1;149(3):603-612.
3.
Figure 5

Figure 5. From: Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis.

GFP-golgin-160 and GFP-golgin-160(D59A) localize to the Golgi. Stable HeLa lines expressing either GFP-golgin-160 (GFP fused to the NH2 terminus of wild-type golgin-160) or GFP-golgin-160(D59A) (GFP fused to the NH2 terminus of the D59A golgin-160 mutant) were generated. Cells were fixed, permeabilized, double stained with anti-GM130 and anti-GFP, and examined by confocal immunofluorescence microscopy. GM-130 antibodies were visualized with goat anti–mouse IgG and assigned the color red (a and d), whereas GFP antibodies were visualized with fluorescein-conjugated goat anti–rabbit and assigned the color green (b and e). When images were merged, overlapping red and green pixels appeared orange/yellow (c and f). Experiments were repeated on four separate occasions with similar results. Bars, 10 μm.

Marie Mancini, et al. J Cell Biol. 2000 May 1;149(3):603-612.
4.
Figure 8

Figure 8. From: Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis.

Golgin-160 cleavage at D59 by caspase-2 contributes to the disintegration of the Golgi complex during apoptosis. The stable HeLa cell lines expressing GFP-golgin-160 (wt) or GFP-golgin-160(D59A) were left untreated or incubated with 1 μm staurosporine (STS) for 2.5 h. (a) GFP-golgin-160–expressing cells from these four groups were assessed for their Golgi morphology (intact versus scattered), and plotted as a percentage of total counted cells. Error bars represent the SD. **P = 0.0028 as determined by the t test (comparing percentages of scattered Golgi in staurosporine-treated wt versus staurosporine-treated D59A). (b) Representative cell with intact Golgi. (c) Representative cells with scattered Golgi. Bar, 10 μm.

Marie Mancini, et al. J Cell Biol. 2000 May 1;149(3):603-612.
5.
Figure 4

Figure 4. From: Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis.

Kinetics of golgin-160 cleavage during apoptosis. (a) HeLa lysates were made at 1, 2, 4, and 6 h after addition of 1 μm staurosporine, and were run on a 7.5% (top) or 10% (bottom) SDS-PAGE. 70 μg of lysate was loaded in each lane. Golgin-160 and PARP were detected by immunoblotting with anti–golgin-160(648) or patient serum recognizing PARP, respectively. Fragments are marked on the right. (b) Graph showing a decrease of the p163 fragment concomitant with an increase in the p140 fragment. Percentage of maximum fragment plotted against time in staurosporine demonstrates that the caspase-2–generated p163 band reaches a maximum at 2 h, whereas the Group II caspase-generated p140 band reaches its maximum at 4 h in staurosporine. The kinetics of the formation of the p140 fragment of golgin-160 is very similar to that for the p89 fragment of PARP.

Marie Mancini, et al. J Cell Biol. 2000 May 1;149(3):603-612.
6.
Figure 3

Figure 3. From: Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis.

Caspases-2, -3, and -7 cleave golgin-160 in vitro at unique sites and generate distinct fragments. [35S]methionine-labeled golgin-160 was expressed using IVTT, and was incubated for 1 h at 37°C with purified caspases-2, -3, or -7. Intact and cleaved golgin-160 were detected by autoradiography. (a) Cleavage with caspase-2 generated a predominant fragment of 163 kD and another of 140 kD. Mutation of aspartic acid to alanine at the amino acid position 59 (D59A) specifically abolished the generation of the unique 163-kD caspase-2–generated fragment, and the D311A mutation abolished generation of the p140 fragment. (b) Caspase-3 cleaved golgin-160 to generate a predominant band of 155 kD and another of 140 kD. The unique p155 fragment was absent in the D139A mutant, and the p140 fragment was absent in the D311A mutant. (c) Cleavage of golgin-160 with caspase-7 generated a single fragment of 140 kD, which was absent in the D311A mutant. (d) Schematic depiction of cleavage of golgin-160 in vitro by caspases-2, -3, and -7. These results are representative of four separate experiments.

Marie Mancini, et al. J Cell Biol. 2000 May 1;149(3):603-612.
7.
Figure 1

Figure 1. From: Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis.

Caspase-2 is localized to the nucleus and Golgi in HeLa cells. HeLa cells (a–f) or HeLa cells stably expressing GFP-golgin-160 (g–i) were fixed, permeabilized, and double-labeled with anti–caspase-2 (a, d, and g) and antigiantin (b and e) or anti-GFP (h). The stained cells were examined by confocal immunofluorescence microscopy. When red (caspase-2) and green (giantin or GFP) images were merged, overlapping red and green pixels appeared orange/yellow (c, f, and i). An identical caspase-2 staining pattern was observed using several different anti–caspase-2 antibodies: affinity-purified MF386, a polyclonal rabbit antiserum raised against the large subunit of caspase-2 (a–c); sc-623, a polyclonal rabbit antiserum raised against an NH2-terminal peptide of caspase-2 precursor (d–f); and anti-ICH-1L, an mAb raised against a peptide encompassing residues 225–401 of caspase-2 (g–i). MF388, a second rabbit antiserum raised against the large subunit of caspase-2, also showed identical staining (data not shown). The experiments were repeated on 11 (a–c), 5 (d–f), or 3 (g–i) separate occasions with identical results. Bars: (a–c and g-i) 10 μm; 25 μm (d–f).

Marie Mancini, et al. J Cell Biol. 2000 May 1;149(3):603-612.
8.
Figure 6

Figure 6. From: Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis.

Golgin-160 is cleaved at D59 in vivo during staurosporine-induced apoptosis. (a) The stable HeLa lines expressing GFP-golgin-160 or GFP-golgin-160(D59A) were incubated with 1 μm staurosporine for 4 h. Lysates from untreated and treated cells were run on 7.5% SDS-PAGE and immunoblotted with rabbit anti-GFP. Incubation with staurosporine resulted in a decrease of the intact GFP-golgin-160 molecule in both wt and D59A mutant cells. A 38-kD fragment was detected only in staurosporine-treated golgin-160 wild-type cells and not in the staurosporine-treated golgin-160(D59A) mutant line, which instead showed an increased level of the p47 fragment. (b) The GFP-golgin-160 and GFP-golgin-160(D59A) stable HeLa lines were labeled with [35S]Met/Cys for 1 h and chased for 8 h with or without 1 μm staurosporine for different periods of time. GFP-containing fragments of golgin-160 were immunoprecipitated with rabbit anti-GFP from lysates from both untreated and staurosporine-treated cells. Staurosporine treatment resulted in the disappearance of intact GFP-golgin-160 in both wt (4 h) and D59A (6 h). A p38 fragment was detected only in staurosporine-treated GFP-golgin-160 wt cells and not in staurosporine-treated GFP-golgin-160(D59A) mutant cells. In contrast, a single fragment at 47 kD was immunoprecipitated from these D59A mutant cells. At the top is a schematic depiction showing the predicted sizes of NH2-terminal fragments of GFP-golgin-160 when cleavage occurs at the unique caspase-2 and caspase-3 cleavage sites. Experiments were performed on three (a) or five (b) separate occasions with similar results.

Marie Mancini, et al. J Cell Biol. 2000 May 1;149(3):603-612.

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