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Results: 5

1.
FIG. 2

FIG. 2. From: Expression and Functional Analysis of Uch-L3 during Mouse Development.

Alignment of the mouse (m.) UCH-L3 amino acid sequence with human (h.) Uch-L3 and mouse UCH-L1. Identical residues are boxed and darkly shaded, and conserved changes are boxed and lightly shaded. Dashes indicate gaps relative to mUCH-L3.

Laurie Jo Kurihara, et al. Mol Cell Biol. 2000 April;20(7):2498-2504.
2.
FIG. 1

FIG. 1. From: Expression and Functional Analysis of Uch-L3 during Mouse Development.

Uch-L3 gene sequence and structure. Exon (Ex) boundaries are denoted above the nucleotide sequence. The exon 4-exon 5 boundary was ambiguous, as indicated. The start and stop codons and residues deleted in Uch-L3Δ3-7 are underlined; the caret denotes conserved cysteine 95.

Laurie Jo Kurihara, et al. Mol Cell Biol. 2000 April;20(7):2498-2504.
3.
FIG. 4

FIG. 4. From: Expression and Functional Analysis of Uch-L3 during Mouse Development.

Uch-L3 expression in adult tissues. Total RNAs from the tissues indicated were hybridized to both Uch-L3 and β-actin probes. The order of the lanes, from left to right, is thymus, gut, lung, liver, spleen, kidney, testis, heart, tongue, brain, and placenta. Based on ethidium bromide staining of rRNA bands, relatively equivalent amounts of RNA were loaded in each lane (data not shown).

Laurie Jo Kurihara, et al. Mol Cell Biol. 2000 April;20(7):2498-2504.
4.
FIG. 3

FIG. 3. From: Expression and Functional Analysis of Uch-L3 during Mouse Development.

Analysis of Uch-L3 transcripts by in situ hybridization. (A) An embryonic day 8.5 (e8.5) embryo is stained at the open edge of the anterior and posterior neural folds (arrow). Staining throughout the embryo was also detected. (B) An e9.5 embryo shows staining at the rim of the posterior neuropore (arrow). (C) An e10.5 embryo is stained at the branchial arches (arrowhead), apical ectodermal ridge (arrow), somites, and tail bud. (D) An e10.5 embryo hybridized with the control sense Uch-L3 probe shows no staining.

Laurie Jo Kurihara, et al. Mol Cell Biol. 2000 April;20(7):2498-2504.
5.
FIG. 5

FIG. 5. From: Expression and Functional Analysis of Uch-L3 during Mouse Development.

Construction of a targeted Uch-L3Δ3-7 allele. (a) The genomic structure of Uch-L3 is indicated in the top line. The wild-type allele depicts the SpeI (gray box) and HindIII (hatched box) fragments used as targeting arms flanking exons 3 to 7. The wild-type allele was detected as a 7.5-kb HindIII fragment with the 5′ probe and as an 11-kb PstI fragment with the 3′ probe. The arrows indicate the primers used to detect the wild-type allele by PCR. The Uch-L3Δ3-7 allele depicts the replacement of exons 3 to 7 with PGK-NEO following targeting. The targeted allele was detected as a 5.5-kb HindIII fragment with the 5′ probe and as an 8-kb PstI fragment with the 3′ probe. The arrows indicate the primers used to detect the targeted allele by PCR. (b) Southern blot hybridization of the 5′ and 3′ probes to wild-type (+/+) and heterozygous (+/Δ) mouse DNAs digested with HindIII (5′) or PstI (3′) to detect the wild-type and targeted restriction fragments. (c) Total RNAs from wild-type (+/+) and Uch-L3Δ3-7 (Δ/Δ) testes were hybridized to Uch-L3 and β-actin probes.

Laurie Jo Kurihara, et al. Mol Cell Biol. 2000 April;20(7):2498-2504.

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