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1.
Figure 4.

Figure 4. From: Surface Antigen Expression and Complement Susceptibility of Differentiated Neuroblastoma Clones.

Western blot analysis of DAF expression by tumor cell lines. Membrane preparations from the indicated neuroblastoma cell lines and the SKOV3 ovarian cancer cell line were analyzed by Western blot using anti-DAF monoclonal antibody 1H4.

Shaohua Chen, et al. Am J Pathol. 2000 March;156(3):1085-1091.
2.
Figure 2.

Figure 2. From: Surface Antigen Expression and Complement Susceptibility of Differentiated Neuroblastoma Clones.

Lysis of anti-GD2-sensitized HTB-63 and NMB-7 by human complement. Cells were sensitized to complement by preincubation in anti-GD2 3F8 monoclonal antibody at 15 μg/ml. Sensitized cells were washed, exposed to the indicated concentration of human serum (37°C/60 minutes), and cell lysis determined. ▶ shows representative data from three separate experiments.

Shaohua Chen, et al. Am J Pathol. 2000 March;156(3):1085-1091.
3.
Figure 7.

Figure 7. From: Surface Antigen Expression and Complement Susceptibility of Differentiated Neuroblastoma Clones.

Surface expression of complement inhibitory proteins on neuroblastoma tumor cell lines. Cells were stained by immunofluorescence using monoclonal antibodies to human CD59 (YTH53.1), MCP (M75), and DAF (1H4) as primary antibodies. ▶ shows relative mean fluorescence by flow cytometric analysis.

Shaohua Chen, et al. Am J Pathol. 2000 March;156(3):1085-1091.
4.
Figure 3.

Figure 3. From: Surface Antigen Expression and Complement Susceptibility of Differentiated Neuroblastoma Clones.

Surface expression of complement inhibitory proteins on tumor cell lines. Cells were stained by immunofluorescence using monoclonal antibodies to human CD59 (YTH53.1), MCP (M75), and DAF (1H4) as primary antibodies. ▶ shows relative mean fluorescence by flow cytometric analysis. Isotype-matched antibodies of irrelevant specificity were used for controls.

Shaohua Chen, et al. Am J Pathol. 2000 March;156(3):1085-1091.
5.
Figure 5.

Figure 5. From: Surface Antigen Expression and Complement Susceptibility of Differentiated Neuroblastoma Clones.

Surface expression of tumor-associated antigens on neuroblastoma tumor cell lines. Cells were stained by immunofluorescence using monoclonal antibodies to GD2 (monoclonal antibody 3F8) and an undefined tumor-selective antigen (monoclonal antibody 8H9) as primary antibodies. ▶ shows relative mean fluorescence by flow cytometric analysis. Isotype-matched antibodies of irrelevant specificity were used for controls.

Shaohua Chen, et al. Am J Pathol. 2000 March;156(3):1085-1091.
6.
Figure 6.

Figure 6. From: Surface Antigen Expression and Complement Susceptibility of Differentiated Neuroblastoma Clones.

Lysis of neuroblastoma tumor cell lines by human complement. Cells were sensitized to complement by preincubation with 8H9 and anti-IgG1 antibodies. Sensitized cells were then exposed to the indicated concentration of human serum (37°C/60 minutes), and cell lysis determined. Either the omission of sensitizing antibodies or the use of heat-inactivated human serum in cell lysis assays resulted in a background lysis <10% of test value. ▶ shows representative data from three separate experiments.

Shaohua Chen, et al. Am J Pathol. 2000 March;156(3):1085-1091.
7.
Figure 1.

Figure 1. From: Surface Antigen Expression and Complement Susceptibility of Differentiated Neuroblastoma Clones.

Lysis of tumor cell lines by human complement. Cells were sensitized to complement by preincubation in 10% anti-membrane rabbit antiserum. Sensitized cells were washed, exposed to the indicated concentration of human serum (37°C/60 minutes), and cell lysis determined. Either the omission of sensitizing antibody or the use of heat-inactivated human serum in cell lysis assays resulted in a background lysis of <10% of test value. Figure ▶ shows representative data from three separate experiments.

Shaohua Chen, et al. Am J Pathol. 2000 March;156(3):1085-1091.
8.
Figure 8.

Figure 8. From: Surface Antigen Expression and Complement Susceptibility of Differentiated Neuroblastoma Clones.

Effect of blocking the function of complement inhibitory proteins on complement-mediated lysis of 5S and 55N cells. 5S cells (a) or 55N cells (b) were preincubated with F(ab)2 fragments of anti-CD59, anti-DAF, or whole IgG anti-MCP monoclonal antibody at 50 μg/ml. Cells were then sensitized to complement, exposed to the indicated concentration of human serum (37°C/60 minutes), and cell lysis determined. Increasing the concentration of function blocking anti-complement inhibitor F(ab)2 fragments or antibody did not further enhance complement-mediated lysis. ▶ shows representative data from three experiments.

Shaohua Chen, et al. Am J Pathol. 2000 March;156(3):1085-1091.

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