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1.
Figure 2

Figure 2. From: Exogenous Expression of N-Cadherin in Breast Cancer Cells Induces Cell Migration, Invasion, and Metastasis.

N-cadherin promotes coaggregation of N-cadherin–transfected MCF-7 cells with N-cadherin–transfected L-cells. L-cells and L-E-cadherin and L-N-cadherin cells, labeled with the fluorescent dye diO (green) were coaggregated with diI-labeled (red) MCF-7 cells (A, B, and C, respectively) or N-cad-5 cells (D, E, and F respectively). Yellow appears when green and red are superimposed, indicating coaggregation.

Rachel B. Hazan, et al. J Cell Biol. 2000 February 21;148(4):779-790.
2.
Figure 10

Figure 10. From: Exogenous Expression of N-Cadherin in Breast Cancer Cells Induces Cell Migration, Invasion, and Metastasis.

N-cadherin promotes adhesion of MCF-7 cells to HUVEC cells. (A) Each panel shows an unstained (and therefore not visible) monolayer of HUVEC cells incubated with control Neo-5 (A), N-cad-5 (B), or N-cad-17 (C) cells labeled with fluorescent dye (Fast diO) (see Materials and Methods). D represents N-cadherin immunoblotting of cell extracts from HUVEC (right) compared with Neo-5 (left) and N-cad-5 (middle) control cells.

Rachel B. Hazan, et al. J Cell Biol. 2000 February 21;148(4):779-790.
3.
Figure 7

Figure 7. From: Exogenous Expression of N-Cadherin in Breast Cancer Cells Induces Cell Migration, Invasion, and Metastasis.

E- and N-cadherin expression in metastatic lesions. Sections of salivary glands (Sal, top left), pancreas (top right); axillary lymph nodes (LN, bottom right), from mice injected with N-cad-5 and N-cad-17 (Table ) were stained with N- and E-cadherin antibodies using a secondary DAB detection. The metastatic cells (mets) express high levels of both N- and E-cadherin. Staining of primary tumors from MCF-7–injected mice (bottom left) shows a positive reaction with E-cadherin but not with N-cadherin antibodies. Bar, 20 μm.

Rachel B. Hazan, et al. J Cell Biol. 2000 February 21;148(4):779-790.
4.
Figure 9

Figure 9. From: Exogenous Expression of N-Cadherin in Breast Cancer Cells Induces Cell Migration, Invasion, and Metastasis.

FGF-2 stimulates Matrigel invasion of N-cadherin–expressing cells and expression of MMP-9. (A) Invasion through Matrigel-coated filters of control and N-cadherin–expressing cells, pretreated for 24 h with 10 ng/ml of FGF-2 or left untreated, as measured 5 h after inoculation into Transwell chambers. The invading cells were stained (see Materials and Methods) and photographed using a digital microscope camera. Each panel illustrates a sample representing three to six filters. (B) Gelatinolytic activity of conditioned media of control (Neo-mass) (lanes 1 and 2) or N-cadherin–transfected MCF-7 cells (N-cad-mass, N-cad-5, and N-cad-17; lanes 3–8) that were either treated with FGF-2, or left untreated, as indicated. (C) Gelatinolytic activity of conditioned media of Neo-mass, N-cad-mass, N-cad-5, and N-cad-17 that were pretreated for 24 h either with 10 ng/ml of FGF-2 (lanes 1, 3, 5, and 7, respectively) or 5 μg/ml insulin (lanes 2, 4, 6, and 8, respectively).

Rachel B. Hazan, et al. J Cell Biol. 2000 February 21;148(4):779-790.
5.
Figure 5

Figure 5. From: Exogenous Expression of N-Cadherin in Breast Cancer Cells Induces Cell Migration, Invasion, and Metastasis.

Histopathology of metastatic lesions in nude mice. 5-μm sections of the pancreas of mice injected with N-cad-17 cells were stained with either H&E (A) or cytokeratin antibodies, followed by DAB detection (B). Cytokeratin immunoreactivity of the N-cad-17 primary tumor (C) was compared with the pancreatic lesion produced by this tumor in B. Sections from the salivary gland of mice injected with N-cad-5 cells were stained with anticytokeratin antibodies, followed by DAB detection (D) and sections from the omentum (E) and primary tumor of N-cad-5–injected mice (F) were stained with FITC-conjugated cytokeratin antibodies. N-cad-8 metastatic lesions were detected in lung sections by H&E (G) and cytokeratin/DAB detection (H). N-cad-15 cells were found in the lumbar spinal muscle (I) using double fluorescent staining with antiactin, followed by secondary antibodies coupled to rhodamine (red) and FITC-conjugated anticytokeratin antibodies (green). Bar: (A) 100 μm; (G and H) 50 μm; (B, D, and E) 33 μm; (C, F, and I) 20 μm.

Rachel B. Hazan, et al. J Cell Biol. 2000 February 21;148(4):779-790.

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