Results: 4

1.
Figure 1

Figure 1. From: Testosterone reduces neuronal secretion of Alzheimer's ?-amyloid peptides.

Schematic diagram of β-amyloid. The amino acid sequence of human Aβ (box), the major known sites of Aβ cleavage and of secretase actions (α, β, and γ), and the antibodies used (22C11 directed at the N-terminal region of βAPP; 6E10 directed at human Aβ1–10; 3431N directed at the N-terminal region of rodent Aβ; 4G8 directed at Aβ17–24; and 369 directed at the C-terminal region of βAPP) are shown.

Gunnar K. Gouras, et al. Proc Natl Acad Sci U S A. 2000 February 1;97(3):1202-1205.
2.
Figure 4

Figure 4. From: Testosterone reduces neuronal secretion of Alzheimer's ?-amyloid peptides.

Immunoprecipitation/mass spectrometry analyses of Aβ release into conditioned medium. Neurons were incubated in the absence or presence of 200 nM testosterone for 10 days. Levels of Aβx-40 and Aβx-42 species measured in the presence of testosterone (Lower) were significantly lower than control (Upper) levels in each of three experiments, including the one illustrated here.

Gunnar K. Gouras, et al. Proc Natl Acad Sci U S A. 2000 February 1;97(3):1202-1205.
3.
Figure 3

Figure 3. From: Testosterone reduces neuronal secretion of Alzheimer's ?-amyloid peptides.

Effect of testosterone and other steroids on secretion of sβAPPα and Aβ from primary cultures of embryonic rat cerebrocortical neurons. (a) Representative autoradiographic analysis of sβAPPα (Lower) and Aβ (Upper). (b) Effects of steroids on the release of sβAPPα (Left) and Aβ (Right) in neurons represented as means ± SD, n = 3–4 (statistically different from control; *, P < 0.01; **, P < 0.03). Effects of 17β-estradiol and testosterone were not statistically different from each other at either 200 nM or 1 μM. (c) Quantitation of Aβ release in response to treatment with 200 nM testosterone for 0–10 days represented as means ± SD, n = 3 (statistically different from control cells incubated for the same duration in the absence of hormone; *, P < 0.03).

Gunnar K. Gouras, et al. Proc Natl Acad Sci U S A. 2000 February 1;97(3):1202-1205.
4.
Figure 2

Figure 2. From: Testosterone reduces neuronal secretion of Alzheimer's ?-amyloid peptides.

Effect of testosterone and other steroids on secretion of sβAPPα and Aβ from murine N2a neuroblastoma cells transfected with human βAPP695. (a) Representative autoradiographic analysis of sβAPPα (Lower) and Aβ (Upper). (b) Effects of steroids on the release of sβAPPα (Left) and Aβ (Right) represented as means ± SD, n = 3 (statistically different from controls by Student's t test; *, P < 0.01; **, P < 0.02). Effects of 17β-estradiol and testosterone were not statistically different from each other at either 200 nM or 1 μM. (c) Quantitation of Aβ release in response to treatment with 200 nM testosterone for 0–14 days. Data are represented as means ± SD, n = 3 (statistically different from control cells incubated for the same duration in the absence of hormone; *, P < 0.02).

Gunnar K. Gouras, et al. Proc Natl Acad Sci U S A. 2000 February 1;97(3):1202-1205.

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