We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 4

1.
Fig. 4

Fig. 4. Hypothetical nanoscale membrane rippling within the lateral wall of the OHC. From: Voltage- and Tension-Dependent Lipid Mobility in the Outer Hair Cell Plasma Membrane.

The plasma membrane is tethered to a subplasmalemmal cytoskeleton by 30-nm-long “pillars” (p) (24). The molecular composition of the pillars is unknown. The pillars bond to parallel actin filaments (a) that run circumferentially around the cell. The actin filaments are spaced about 40 nm apart and cross-linked with molecules of spectrin (s) that run longitudinally along the cell. (A and C) The OHC when hyperpolarized and depolarized, respectively. Depolarization makes the OHC shorter and wider. (B and D) Potential alterations in membrane curvature resulting from electromotile length changes. Note the increased membrane crenulations in (D).

John S. Oghalai, et al. Science. ;287(5453):658-661.
2.
Fig. 3

Fig. 3. The effects of curvature-altering drugs on OHC electromotility. From: Voltage- and Tension-Dependent Lipid Mobility in the Outer Hair Cell Plasma Membrane.

The percent change in cell length(L)was measured as the holding potential was varied, normalized to cell length at −60 mV. The control cell (cntl) demonstrated the normal electromotile response. The presence of chlorpromazine alone (cpz) did not suppress electromotility. Salicylate alone (sal) blunted the electromotile response. Chlorpromazine failed to restore electromotility in the presence of salicylate (both). Each curve was obtained from a different cell. The Boltzmann fit values are: cntl, Lhyperpolarized = 2.25%, Ldepolarized = −5.64%, V1/2 = −19 mV, s = 42 mV; sal, Lhyperpolarized = 0.39%, Ldepolarized = −0.65%, V1/2 = −35 mV, s = 41 mV; cpz, Lhyperpolarized = 2.10%, Ldepolarized = −6.73%, V1/2 = −17 mV, s = 37 mV; both, Lhyperpolarized = 0.91%, Ldepolarized = −0.81%, V1/2 = −58 mV, s = 59 mV.

John S. Oghalai, et al. Science. ;287(5453):658-661.
3.
Fig. 2

Fig. 2. The effects of curvature-altering drugs on cell morphology and lateral diffusion. From: Voltage- and Tension-Dependent Lipid Mobility in the Outer Hair Cell Plasma Membrane.

The RBCs serve as a bioassay for the curvature-altering drugs. (A) RBCs were biconcave in the control solution, (B) crenulated in salicylate alone, (C) biconcave in a solution of salicylate and chlorpromazine combined, (D) cupped in chlorpromazine alone, and (E) biconcave after wash-out with control solution. During this 5-min sequential drug application, the length, width, and morphologic appearance of the OHC did not change appreciably. (F) The D value of the OHC lateral wall plasma membrane was reduced from controls (cntl) in the presence of either salicylate alone (sal) or chlorpromazine alone (cpz). It was unchanged in the presence of salicylate and chlorpromazine combined (both) (P = 0.38). The values in parentheses indicate the number of cells averaged together for each measurement and the error bars represent the SEM; a significant difference from control is indicated by an asterisk (P < 0.01, Student’s nonpaired t-test). The D values were as follows (mean ± SEM): cntl, 5.46 × 10−9 ± 0.44 × 10−9 cm2/s; sal, 2.58 × 10−9 ± 0.25 × 10−9 cm2/s; cpz, 3.64 × 10−9 ± 0.32 × 10−9 cm2/s; both, 4.83 × 10−9 ± 0.41 × 10−9 cm2/s.

John S. Oghalai, et al. Science. ;287(5453):658-661.
4.
Fig. 1

Fig. 1. Lateral diffusion in the OHC lateral wall plasma membrane. From: Voltage- and Tension-Dependent Lipid Mobility in the Outer Hair Cell Plasma Membrane.

Each point is the average of at least five different measurements, each from a different cell. The error bars represent the SEM. (A) D was measured at holding potentials ranging from +40 to −100 mV (total of 70 measurements from 41 cells). The error bar for the point at 40 mV is smaller than the symbol size. The Boltzmann fit values are: Ddepolarized = 2.18 × 10−9 cm2/s, Dhyperpolarized = 4.44 × 10−9 cm2/s, V1/2 = −36 mV, s = 12 mV (23).(B) D was measured while the osmolality of the extracellular solution was varied between 245 and 365 mOsm/kg (total of 275 cells, one measurement per cell). The error bar for the point at 300 mOsm/kg is smaller than the symbol size. The Boltzmann fit values are: Dhypotonic = 3.70 × 10−9 cm2/s, Dhypertonic = 6.34 × 10−9 cm2/s, Osm1/2 (the osmolality at the midpoint of the change in D) = 316 mOsm/kg, s = 20 mOsm/kg. The slightly higher D values in these cells compared with those in (A) may be related to differences in intracellular pressure associated with the patch-clamp technique (17).(C) The percent change in cell length (L) was measured while the osmolality of the extracellular solution was varied. This was normalized to their length at 300 mOsm/kg (total of 154 measurements from 77 cells). The Boltzmann fit values are: Lhypotonic = −5.79%, Lhypertonic = 3.51%, Osm1/2 = 294 mOsm/kg, s = 13 mOsm/kg.(D) D was plotted versus L [with the same data as (B) and (C)]. The relation was fit with an exponential function, D = D0 + Aexp(RL), with fit values D0 = 3.43 × 10−9 cm2/s, A = 1.21 × 10−9 cm2/s, R = 0.21.

John S. Oghalai, et al. Science. ;287(5453):658-661.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk