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Results: 3

1.
Figure  1

Figure 1. From: Microsatellite Polymorphism in the Heme Oxygenase-1 Gene Promoter Is Associated with Susceptibility to Emphysema.

a, Nucleotide sequence of the 5′-flanking region and exon 1 of the human HO-1 gene (X14782) (Shibahara et al. 1989; GenBank). The fragment between P1-s and P1-as was amplified by PCR and the number of (GT)n repeats was determined by a DNA sequencer. b, Frequency distribution of the numbers of (GT)n repeats in patients without CPE (non-CPE; n=100), patients with CPE (CPE;, n=101), and total patients (Total: n=201).

Norihiro Yamada, et al. Am J Hum Genet. 2000 January;66(1):187-195.
2.
Figure  2

Figure 2. From: Microsatellite Polymorphism in the Heme Oxygenase-1 Gene Promoter Is Associated with Susceptibility to Emphysema.

Northern-blot analysis demonstrating increases in HO-1 mRNA levels in A549 cells (a) and Hep3B cells (b), before (0 h) and 1, 3, 5, and 7 h after treatment with either 100, 200, 500, or 1,000 μM of H2O2. β-Actin was used as an internal control. HO-1 mRNA was normalized with a constitutive expression of β-actin mRNA. The ratio of each normalized value to that of the 0-time control is shown as a relative expression level of HO-1 mRNA (HO-1/β-actin). Data are representative of three different experiments.

Norihiro Yamada, et al. Am J Hum Genet. 2000 January;66(1):187-195.
3.
Figure  3

Figure 3. From: Microsatellite Polymorphism in the Heme Oxygenase-1 Gene Promoter Is Associated with Susceptibility to Emphysema.

Promoter activities of 5′-flanking regions of the HO-1 gene in either A549 cells (a) or Hep3B cells (b) transfected with the recombinant gene carrying either (GT)16, (GT)20, (GT)29, or (GT)38 repeat after treatment with either H2O2 (500 μM for 3 h) or distilled water (control), and the effects of catalase (2,000 U/ml for 15 min) on the increase in relative luciferase activity induced by H2O2 in the A549 cells transfected with the gene carrying (GT)16. Each luciferase activity was normalized to Renilla luciferase activity and was expressed as a ratio to the control value of the cells with (GT)38. Results are reported as mean ± SEM from nine samples. Significant increases by H2O2 are indicated by * (P<.05) and *** (P<.001), respectively. Significant decrease by catalase is indicated by + (P<.05). Significant differences in basal promoter activity are indicated by § (P<.05).

Norihiro Yamada, et al. Am J Hum Genet. 2000 January;66(1):187-195.

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