Results: 4

1.
Figure 3

Figure 3. From: Pax6 is essential for establishing ventral-dorsal cell boundaries in pituitary gland development.

Ventral markers are overexpressed in the absence of Pax6 in the determined pituitary gland. At e15, the PFrk, GATA2, and SF-1 (a gonadotrope-specific marker) expression levels are increased in the absence of Pax6.

Chrissa Kioussi, et al. Proc Natl Acad Sci U S A. 1999 December 7;96(25):14378-14382.
2.
Figure 2

Figure 2. From: Pax6 is essential for establishing ventral-dorsal cell boundaries in pituitary gland development.

Ventral markers are overexpressed in the absence of Pax6 in the early stage of pituitary development. At e12 in Rathke’s pouch, the ventral markers αGSU, GATA2, Brn4, Isl-1, and Prop-1 are overexpressed in the Pax6 mutant mice. No difference has been observed for the POMC and Nkx3.1 levels. Pitx1, a uniformly expressed pituitary marker, showed an up-regulation at the ventral site of the pouch.

Chrissa Kioussi, et al. Proc Natl Acad Sci U S A. 1999 December 7;96(25):14378-14382.
3.
Figure 1

Figure 1. From: Pax6 is essential for establishing ventral-dorsal cell boundaries in pituitary gland development.

Pax6 expression pattern during pituitary gland development. (A) Pax6 transcripts are detected very early in oral ectoderm at e9.5 as they occupy the Shh-nonexpressing area. Later, at e10–e12, Pax6 is characterized by a dorsalhigh to ventrallow expression pattern along the Rathke’s pouch. Pax6 transcripts are diminished during embryonic development, and no signal is detected before birth. (B) Pax6 protein is detected at the dorsal pituitary gland at e11.5. Pax6-positive cells are located only in the lumen at e14.5. (C) Schematic representation of Pax6 expression in combination with the ventral and dorsal signaling molecules and transcription factors. By e12.5, Pax6 expression is excluded from the most ventral cell types, the gonadotropes and thyrotropes. MSH, melanocyte-stimulating hormone; C, corticotropes; S/L, somatotropes/lactotropes; T, thyrotropes; G, gonadotropes; Tr, rostral tip thyrotropes.

Chrissa Kioussi, et al. Proc Natl Acad Sci U S A. 1999 December 7;96(25):14378-14382.
4.
Figure 4

Figure 4. From: Pax6 is essential for establishing ventral-dorsal cell boundaries in pituitary gland development.

Ventral pituitary cell types are increased in the absence of Pax6. (A) Newborn Pax6 mutant mice are characterized by increased GH, TSH, and αGSU as well as by down-regulation of LH but not by a difference in POMC levels. (B) Very few dorsal GH- and PRL-positive cells are detected in mutant mice. (C) Pax6 establishes the boundary along the dorsoventral pituitary axis as early as e10 in response to the inhibitory Shh gradient and maybe the BMP2 from the ventral Rathke’s pouch and oral ectoderm. In the absence of Pax6, there is a dorsal shift of the boundary, with up-regulation of the transcription factors determining the most ventral cell types, the gonadotropes and thyrotropes. M, melanotropes; other abbreviations are defined in the legend to Fig. .

Chrissa Kioussi, et al. Proc Natl Acad Sci U S A. 1999 December 7;96(25):14378-14382.

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