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Results: 3

1.
FIG. 3

FIG. 3. From: Growth Phase-Dependent Variation in Protein Composition of the Escherichia coli Nucleoid.

Growth phase-dependent variation in the intracellular levels of 12 DNA-binding proteins in E. coli W3110. The measurement of each DNA-binding protein was carried out as described in Materials and Methods. (A) Total numbers of each protein molecule at three distinct growth phases are shown. Exponential phase, 2.3 to 3.0 h; early stationary phase, 5.0 h; late stationary phase, 48 h (see Fig. 1 for the growth curve). (B) The order of the total number of molecules among the 12 DNA-binding proteins.

Talukder Ali Azam, et al. J Bacteriol. 1999 October;181(20):6361-6370.
2.
FIG. 2

FIG. 2. From: Growth Phase-Dependent Variation in Protein Composition of the Escherichia coli Nucleoid.

Growth phase-dependent variation in the intracellular levels of 12 DNA-binding proteins in E. coli W3110. For measurement of the concentrations of each DNA-binding protein, aliquots of cell lysates containing 15 μg of total proteins were subjected to SDS-PAGE (with a 10% gel for DnaA; a 12.5% gel for CbpA, CbpB, and IciA; and a 16.5% gel for Dps, Fis, Hfq, H-NS, HU, IFH, Lrp, and StpA) and proteins were electroblotted onto polyvinylidene difluoride membranes. The blots were probed with anti-CbpA (A), anti-CbpB or anti-Rob (B), anti-DnaA (C), anti-Dps (D), anti-Fis (E), anti-Hfq (F), anti-H-NS (G), anti-HU (H), anti-IciA (I), anti-IHF (J), anti-Lrp (K), or anti-StpA (L) antibodies. After immunostaining, the intensity of stained bands was measured by scanning the gels with an NIH Image analyzer system (version 1.61). To increase the accuracy of the calculation of protein molecules, the Western blot analysis was repeated at least three times for all samples. Solid circles represent the total number of cells measured with a Coulter Multisizer II, while open circles represent the total number of protein molecules per cell, calculated as averages of three independent measurements.

Talukder Ali Azam, et al. J Bacteriol. 1999 October;181(20):6361-6370.
3.
FIG. 1

FIG. 1. From: Growth Phase-Dependent Variation in Protein Composition of the Escherichia coli Nucleoid.

Expression patterns of E. coli W3110 proteins at various growth phases. (A) An early-stationary-phase culture of E. coli W3110 (A type) in LB medium at 37°C was diluted 170-fold with fresh LB medium, and the shaking culture was continued at 37°C. The total number of cells per milliliter (solid circles) and the average volume of cells (open squares) were measured with a Coulter Multisizer II. The protein concentration of cell lysates (open circles) was determined by staining with a Bio-Rad protein assay kit. (B) The same E. coli W3110 (A type) culture for the indicated times was stained with DAPI and observed for cell morphology. The bar in the 2.3-h sample represents 1 μm. (C) At the indicated time points (above the lane markers), aliquots of the culture were removed for the preparation of cell lysates. Portions of the cell lysates containing 15 μg of total proteins were subjected to SDS–15% PAGE. Gels were stained with Coomassie brilliant blue. The molecular mass markers (M, standard markers; PM, prestained markers) used were as follows: phosphorylase b, 97.4 kDa; bovine serum albumin, 66.2 kDa; ovalbumin, 45.0 kDa; carbonic anhydrase, 31.0 kDa; soybean trypsin inhibitor, 21.5 kDa; and lysozyme, 14.4 kDa.

Talukder Ali Azam, et al. J Bacteriol. 1999 October;181(20):6361-6370.

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