PMC

Rapid systemic induction of A70 depends on RPM1 recognition. RNA blots of A70 (At5g56980) accumulation 4 hpi in local or systemic tissues after virulent (D) or avirulent (avrRpm1) (R) P. syringae pv tomato DC3000 challenge. (A) A70 accumulation in RPM1-compromised rpm1 or rar1 mutants or in transgenic plants expressing a bacterial salicylic hydroxylase gene (NahG) (6). (B) Coinfiltration with the calcium channel blocker lanthanum chloride (La³⁺, 1.5 mM), but not the NADPH oxidase inhibitor diphenyl-iodonium (DPI, 7 μM), blocks systemic A70 accumulation. (C) A70 induction in systemic leaves 4 hpi with DC3000(avrRpm1) after inoculated leaves were removed at the time points indicated.

F-measure comparison in scenarios A₉₀, A₇₀, and A₅₀ (uncorrelated predictors). EN, elastic net; LL, logistic lasso; SW, stepwise regression.

The six promising X-to-proline substitutions. The specific positions for E36, A70, A415, V571, D585 and G637 in DgAS are illustrated in A-E, respectively. In F, G637 is highlighted by the CPK model because of its small size.

Fig. 8. The C-terminus of PABPN1 is necessary for stimulation of poly(A) polymerase. Polyadenylation reactions containing radioactively labeled A₇₀ (40 fmol of 3′-ends) and poly(A) polymerase (30 fmol) were incubated with increasing amounts of wild-type or C-terminal truncation variants of PABPN1 as indicated. Lane 1, untreated A₇₀; lane 2, incubation with a 10-fold higher amount of poly(A) polymerase; lanes 3–11, 13–20, 21–28, 29–35, reactions with 0, 100, 200, 500, 1000, 2000, 5000 and 10000 fmol of PABPN1 variants, as indicated. The reaction in the presence of 100 fmol of PABPN1-ΔC27 was left out. Products were analyzed on a denaturing polyacrylamide gel.