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Select item 34254981.

Glycoform and Net Charge Heterogeneity in gp120 Immunogens Used in HIV Vaccine Trials

Bin Yu, Javier F. Morales, Sara M. O'Rourke, Gwen P. Tatsuno, Phillip W. Berman

PLoS One. 2012; 7(8): e43903. Published online 2012 August 22. doi: 10.1371/journal.pone.0043903

PMCID:: PMC3425498

Article PubReader PDF–1.2M

Select item 34128072.

HIV-1 Envelope Resistance to Proteasomal Cleavage: Implications for Vaccine Induced Immune Responses

Nicholas J. Steers, Silvia Ratto-Kim, Mark S. de Souza, Jeffrey R. Currier, Jerome H. Kim, Nelson L. Michael, Carl R. Alving, Mangala Rao

PLoS One. 2012; 7(8): e42579. Published online 2012 August 6. doi: 10.1371/journal.pone.0042579

PMCID:: PMC3412807

Article PubReader PDF–3.8M Supplementary Material

Select item 31964283.

Analysis of a Clonal Lineage of HIV-1 Envelope V2/V3 Conformational Epitope-Specific Broadly Neutralizing Antibodies and Their Inferred Unmutated Common Ancestors

Mattia Bonsignori, Kwan-Ki Hwang, Xi Chen, Chun-Yen Tsao, Lynn Morris, Elin Gray, Dawn J. Marshall, John A. Crump, Saidi H. Kapiga, Noel E. Sam, Faruk Sinangil, Marie Pancera, Yang Yongping, Baoshan Zhang, Jiang Zhu, Peter D. Kwong, Sijy O'Dell, John R. Mascola, Lan Wu, Gary J. Nabel, Sanjay Phogat, Michael S. Seaman, John F. Whitesides, M. Anthony Moody, Garnett Kelsoe, Xinzhen Yang, Joseph Sodroski, George M. Shaw, David C. Montefiori, Thomas B. Kepler, Georgia D. Tomaras, S. Munir Alam, Hua-Xin Liao, Barton F. Haynes

J Virol. 2011 October; 85(19): 9998–10009. doi: 10.1128/JVI.05045-11

PMCID:: PMC3196428

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Select item 35479334.

Analysis of V2 Antibody Responses Induced in Vaccinees in the ALVAC/AIDSVAX HIV-1 Vaccine Efficacy Trial

Susan Zolla-Pazner, Allan C. deCamp, Timothy Cardozo, Nicos Karasavvas, Raphael Gottardo, Constance Williams, Daryl E. Morris, Georgia Tomaras, Mangala Rao, Erik Billings, Phillip Berman, Xiaoying Shen, Charla Andrews, Robert J. O'Connell, Viseth Ngauy, Sorachai Nitayaphan, Mark de Souza, Bette Korber, Richard Koup, Robert T. Bailer, John R. Mascola, Abraham Pinter, David Montefiori, Barton F. Haynes, Merlin L. Robb, Supachai Rerks-Ngarm, Nelson L. Michael, Peter B. Gilbert, Jerome H. Kim

PLoS One. 2013; 8(1): e53629. Published online 2013 January 17. doi: 10.1371/journal.pone.0053629

PMCID:: PMC3547933

Article PubReader PDF–902K

Select item 36483495.

Dynamic electrophoretic fingerprinting of the HIV-1 envelope glycoprotein

Daniel J Stieh, Joshua L Phillips, Paul M Rogers, Deborah F King, Gianguido C Cianci, Simon A Jeffs, Sandrasegaram Gnanakaran, Robin J Shattock

Retrovirology. 2013; 10: 33. Published online 2013 March 20. doi: 10.1186/1742-4690-10-33

PMCID:: PMC3648349

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Select item 34418166.

Extensive glycoform heterogeneity in the gp120 envelope proteins used in the RV144 trial

B Yu, JF Morales, SM O’Rourke, GP Tatsuno, PW Berman

Retrovirology. 2012; 9(Suppl 2): P296. Published online 2012 September 13. doi: 10.1186/1742-4690-9-S2-P296

PMCID:: PMC3441816

Article PubReader PDF–164K

Select item 34862907.

Antibody-Dependent Cellular Cytotoxicity-Mediating Antibodies from an HIV-1 Vaccine Efficacy Trial Target Multiple Epitopes and Preferentially Use the VH1 Gene Family

Mattia Bonsignori, Justin Pollara, M. Anthony Moody, Michael D. Alpert, Xi Chen, Kwan-Ki Hwang, Peter B. Gilbert, Ying Huang, Thaddeus C. Gurley, Daniel M. Kozink, Dawn J. Marshall, John F. Whitesides, Chun-Yen Tsao, Jaranit Kaewkungwal, Sorachai Nitayaphan, Punnee Pitisuttithum, Supachai Rerks-Ngarm, Jerome H. Kim, Nelson L. Michael, Georgia D. Tomaras, David C. Montefiori, George K. Lewis, Anthony DeVico, David T. Evans, Guido Ferrari, Hua-Xin Liao, Barton F. Haynes

J Virol. 2012 November; 86(21): 11521–11532. doi: 10.1128/JVI.01023-12

PMCID:: PMC3486290

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Select item 33838598.

The Thai Phase III Trial (RV144) Vaccine Regimen Induces T Cell Responses that Preferentially Target Epitopes within the V2 Region of HIV-1 Envelope

Mark S. de Souza, Silvia Ratto-Kim, Weerawan Chuenarom, Alexandra Schuetz, Somsak Chantakulkij, Bessara Nuntapinit, Anais Valencia-Micolta, Doris Thelian, Sorachai Nitayaphan, Punnee Pitisuttithum, Robert M. Paris, Jaranit Kaewkungwal, Nelson L. Michael, Supachai Rerks-Ngarm, Bonnie Mathieson, Mary Marovich, Jeffrey R. Currier, Jerome H. Kim, the MOPH-TAVEG collaborators

J Immunol. Author manuscript; available in PMC 2013 May 15.

Published in final edited form as: J Immunol. 2012 May 15; 188(10): 5166–5176. Published online 2012 April 23. doi: 10.4049/jimmunol.1102756

PMCID:: PMC3383859

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Select item 32230899.

RASSF1A and the BH3-only mimetic ABT-737 promote apoptosis in pediatric medulloblastoma cell lines

Jane Levesley, Meryl E. Lusher, Janet C. Lindsey, Steven C. Clifford, Richard Grundy, Beth Coyle

Neuro Oncol. 2011 December; 13(12): 1265–1276. Published online 2011 August 31. doi: 10.1093/neuonc/nor129

PMCID:: PMC3223089

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Select item 239748910.

A Threonine on the Active Site Loop Controls Transition State Formation in Escherichia coli Respiratory Complex II

Thomas M. Tomasiak, Elena Maklashina, Gary Cecchini, Tina M. Iverson

J Biol Chem. 2008 May 30; 283(22): 15460–15468. doi: 10.1074/jbc.M801372200

PMCID:: PMC2397489

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Select item 292031511.

Comparative Immunogenicity of HIV-1 Clade C Envelope Proteins for Prime/Boost Studies

Douglas H. Smith, Peggy Winters-Digiacinto, Misrach Mitiku, Sara O'Rourke, Faruk Sinangil, Terri Wrin, David C. Montefiori, Phillip W. Berman

PLoS One. 2010; 5(8): e12076. Published online 2010 August 11. doi: 10.1371/journal.pone.0012076

PMCID:: PMC2920315

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Select item 344168812.

Antibody responses to V2 loop are induced by CRF01_A E and not Clade B envelopes

N Karasavvas, C Karnasuta, S Madnote, D Arworn, F Sinangil, S Rerks-Ngarm, RJ O'Connell, S Nitayaphan, V Ngauy, JH Kim, NL Michael, MS de Souza

Retrovirology. 2012; 9(Suppl 2): P100. Published online 2012 September 13. doi: 10.1186/1742-4690-9-S2-P100

PMCID:: PMC3441688

Article PubReader PDF–129K

Select item 338545313.

Bad Neighbors: Arsenic-Induced Tumor Cells Convert Normal Stem Cells into a Cancerous Phenotype

Julia R. Barrett

Environ Health Perspect. 2012 June; 120(6): a244. Published online 2012 June 1. doi: 10.1289/ehp.120-a244a

PMCID:: PMC3385453

Article PubReader PDF–162K

Select item 311272514.

Successful development of novel monoclonal antibodies against HTLV-1 bZIP factor and their applications in studying the pathogenesis of HAM/TSP

Mineki Saito, Reiko Tanaka, Akira Kodama, Toshio Matsuzaki, Masahito Suehara, Yuetsu Tanaka

Retrovirology. 2011; 8(Suppl 1): A244. Published online 2011 June 6. doi: 10.1186/1742-4690-8-S1-A244

PMCID:: PMC3112725

Article PubReader PDF–166K

Select item 289887515.

ENDOCRINE HEALTH: Nitrate May Feed Thyroid Disorders

Bob Weinhold

Environ Health Perspect. 2010 June; 118(6): A244.

PMCID:: PMC2898875

Article PubReader PDF–598K

Select item 243025816.

Erin E. Dooley

Environ Health Perspect. 2008 June; 116(6): A244–A245.

PMCID:: PMC2430258

Article PubReader PDF–279K

Select item 144080517.

Fellowships, Grants, & Awards

Environ Health Perspect. 2006 April; 114(4): A244–A245.

PMCID:: PMC1440805

Article PubReader PDF–49K

Select item 156643018.

Testing their metal.

B E Fisher

Environ Health Perspect. 1999 May; 107(5): A244–A245.

PMCID:: PMC1566430

Summary Page Browse PDF–2.3M

Select item 191551919.

Poster Presentations

Am J Hum Genet. 1996 October; 59(Suppl): A244–A296.

PMCID:: PMC1915519

Summary Page Browse PDF–16M

Select item 338545420.

Casting a Wider Net: The Quest for Better Guidance on Seafood Consumption

Kellyn S. Betts

Environ Health Perspect. 2012 June; 120(6): a244. Published online 2012 June 1. doi: 10.1289/ehp.120-a244b

PMCID:: PMC3385454

Article PubReader PDF–162K

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PMC Images search for 10.1289/ehp.119-a244

Figure 2ELISA reactivity of human anti-V2 mAbs 697-D and 2158 with AIDSVAX subtype E (A244, green square) and AIDSVAX subtype B (MN, O) gp120 immunogens.

The pink dashed line represents twice background levels.

Analysis of V2 Antibody Responses Induced in Vaccinees in the ALVAC/AIDSVAX HIV-1 Vaccine Efficacy Trial

PLoS One. 2013;8(1):e53629.
FIG. 5.

PCR analysis of leafhopper DNA (100 ng per reaction). PCR primers derived from clones A10, A182, A244, and A380 (A380 is the same primer as was used for the plant PCR assay for Fig. 4) were used to amplify a segment of DNA extracted from leafhoppers. All reactions except that with A380 primers were nested PCR. Lanes 2, 5, 8, and 11, PCRs with template DNAs from phytoplasma-carrying leafhoppers. Lanes 3, 6, 9, and 12, PCRs with template DNAs from phytoplasma-free leafhoppers. Lanes 1, 4, 7, and 10, positive controls (with the cloned DNAs serving as templates) for PCRs with A182, A244, A380, and A10, respectively.

Identification and Characterization of Phytoplasmal Genes, Employing a Novel Method of Isolating Phytoplasmal Genomic DNA

J Bacteriol. 2003 November;185(22):6513-6521.
Figure 1SDS PAGE analysis of MN-rgp120 and A244-rgp120 HIV produced in CHO and 293 cells.

Recombinant gp120 from the MN and A244 strains of gp120 was expressed in CHO cells and in 293 cells. These were then treated with PNGase (panel A), neuraminidase (panel B), or Endo H (panel C) and resolved on 4–12% SDS-PAGE gels. Plus (+) indicates enzyme treatment; minus (−) indicates untreated.

Glycoform and Net Charge Heterogeneity in gp120 Immunogens Used in HIV Vaccine Trials

PLoS One. 2012;7(8):e43903.
Figure 7Estimated odds ratios (OR) and 95% confidence intervals for each of the V2 assays.

Data are derived from the categorical analyses shown in Table 1. Estimated ORs compare subgroups defined by High vs. Low responses except for comparisons for analyses of IgA V2 A244 K178 and V2 MN which compare Positive vs. Negative responses. For evaluation of biotinylated V2 Peptide 6, comparison is between High and Negative responses.

Analysis of V2 Antibody Responses Induced in Vaccinees in the ALVAC/AIDSVAX HIV-1 Vaccine Efficacy Trial

PLoS One. 2013;8(1):e53629.

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