PMC

¹H NOESY spectrum (tile plot) showing the assignment of exocyclic etheno protons H7 and H6 and NOEs between the etheno and DNA protons. The NOESY spectrum was recorded at 25 °C with a mixing time of 250 ms: (a) X⁶ H7 → X⁶ H6, (b) X⁶ H7 → C¹⁸ H2′, (c) X⁶ H7 → C¹⁸ H2′′, (d) X⁶ H7 → A¹⁹ H5′/H5′′, (e) X⁶ H7 → C¹⁸ H5, (f) X⁶ H7 → C¹⁸ H1′, (g) X⁶ H7 → C¹⁸ H6, (h) X⁶ H7 → A¹⁹ H2, (i) X⁶ H6 → C¹⁸ H2′, (j) X⁶ H6 → C¹⁸ H2′′, (k) X⁶ H6 → A¹⁹ H5′/H5′′, (l) X⁶ H6 → A¹⁹ H5′/H5′′, (m) X⁶ H6 → A¹⁹ H4′, (n) X⁶ H6 → C¹⁸ H1′, (o) X⁶ H6 → A¹⁹ H1′, (p) X⁶ H6 → A¹⁹ H2, (q) X⁶ H6 → C¹⁸ H6, and (r) X⁶ H6 → A¹⁹ H8.

Expansion of the NOESY spectrum (250 ms) of the duplex containing the S-cdG·dA base pair showing the intercalation of A¹⁹ and A²⁰. NOE cross-peaks are assigned as follows: a, T⁶ H6 → T⁶ H1′; b, T⁶ H6 → X⁵ H1′; c, A²⁰ H2 → A²⁰ H1′; d, A²⁰ H2 → T⁶ H1′; e, A²⁰ H2 → A¹⁹ H1′; f, A²⁰ H2 → X⁵ H1′; g, A¹⁹ H2 → A²⁰ H1′; and h, A¹⁹ H2 → A¹⁹ H1′.

Base pair stacking interactions of the cis-(5R,6S) Tg lesion. Comparison stacking interactions in which Tg CH₃ is in the axial (PDB ID 2KH5) or equatorial (PDB ID 2KH6) conformations. The top panel shows the G⁵• C²⁰ base pair (black) stacked above the X⁶• A¹⁹ base pair (Tg is colored red and A¹⁹ is colored gray). The center panel shows the orientation of the X⁶ lesion (red) with respect the complementary nucleotide A¹⁹ (black). The bottom panel shows the X⁶• A¹⁹ base pair (Tg is colored red and A¹⁹ is colored black) stacked above the G⁷• C¹⁸ base pair (grey).

Immunochemical analysis of the LPSs from autoagglutinating Aeromonas strains. (A) Western blot analysis of LPSs reacted with antiserum prepared against purified LPS from strain TF7 (serogroup O:11). The dilution of antiserum used was 1:500. Shown are TF7 (lane 1), AH290 (lane 2), A19 (lane 3), E37 (lane 4), TW1 (lane 5), and E40 (lane 6). (B) Western blot analysis of LPSs reacted with antiserum prepared against heat-killed cells of strain AH290. The dilution of antiserum used was 1:500. Shown are A19 (lane 1), E37 (lane 2), TW1 (lane 3), E40 (lane 4), AH290 (lane 5), and TF7 (lane 6). (C) Western blot analysis of LPSs reacted with antiserum prepared against heat-killed cells of strain A19. The dilution of antiserum used was 1:1,000. Shown are A19 (lane 1), E37 (lane 2), AH290 (lane 3), TF7 (lane 4), TW1 (lane 5), and E40 (lane 6).