PMC

Ribosomal sedimentation profiles of HEK-293 and HTB-14 cells. (Top panels) A₂₅₄ absorption spectra of 10 to 50% sucrose gradients containing lysates generated from mock-infected HEK-293 cells (A), lysates generated from HTB-14 cells (F), or HEK-293 lysates treated with EDTA (D). (Middle panels) A₂₅₄ absorption spectra of lysates generated from PV-RIPO-infected HEK-293 (B), PV-RIPO-infected HTB-14 (G), or PV-infected HEK-293 (E) cells. (C) A₂₅₄ absorption spectra of a 5 to 20% sucrose gradient containing free protein and 40S ribosomal subunit components from a PV-RIPO-infected HEK-293 cell lysate. Lysates from infected cells were generated at 4 hpi. Western blot analyses were performed with α-DRBP76, α-NF45, α-RpS6, and α-tubulin antibodies, as indicated. Viral cDNA corresponding to the IRES region was amplified by RT-PCR, and rRNA was analyzed from total RNA. M, monosome; P, polysome.

Inhibition of KLK6 activity by IgYs. The activity was measured in the absence (A) and presence of IgYs at increasing molar ratios of IgYs:rKLK6 equal to 1:1, 20:1 and 30:1 (B, C and D, respectively). Then, the trypsin substrate N-benzoyl-L-arginine ethyl ester (BAEE) was added and the change in absorbance at A254 was measured relative to time for 3 min. The corresponding changes in the absorbance at A254 were normalized and expressed % of the rKLK6 without addition of the antibodies. IgYs could inhibit rKLK6 only moderately as 61% of its activity remained at a molar ratio as high as 30:1, while the E24 mouse monoclonal control could extinguish 60% of the activity at 1:1. Rates of hydrolysis of the BAEE substrate by rKLK6 are shown. BAEE was used at 250 μΜ and rKLK6 at 12 nM.

HPLC elution profile (A₂₅₄) and radioactivity (disintegrations per minute) of the ethyl acetate-soluble metabolites formed by C. elegans in 4 days from 6-nitro[³H]chrysene. Fractions eluting from the column were collected at 0.5-min intervals, and the radioactivity was measured by liquid scintillation counting. The inset shows the disappearance of 6-nitro[³H]chrysene and formation of ³H-labeled metabolites in cultures of C. elegans.

Percentages of N-phenylpiperazine or N-phenylethylenediamine remaining in cultures of WWTP reactor liquor-inoculated medium with molasses, compared to sterile controls, as shown by HPLC (A₂₅₄). Cultures were dosed with NPP at 100 mg liter⁻¹ (□) and 50 mg liter⁻¹ (▪) or with PED at 100 mg liter⁻¹ (○). Error bars represent standard errors of three samples.