PMC

A. Schematic diagram showing ST3 cleavage sites between the transmembrane domain (TM) and the laminin binding sequence (LB) of LR (27). ST3 cleaves LR between A115 and F116 (site a), and P133 and I134 (site b) and these two cleavage sites by ST3 are indicated by two arrows (Based on (27)).
B. LR mutants used in this study.

C155 location on the crystal structure of MtbFtsZ dimer. (Top panel) The position of C155 in MtbFtsZ. C155 is located between the helix H8 and strand B6. Figure shows the location of GTPγS bound to MtbFtsZ with respect to C155. Cyan and magenta colors show helices and sheets, respectively. Cysteine is shown as yellow spheres and GTPγS as red spheres. (Bottom panel) The environment around Cys-155. Shown as sticks are residues within a sphere of 5.0 Å around the SG atom of Cys-155. Cys-155 side chain atoms shown as yellow spheres are placed in the hydrophobic pocket defined by V98, A115, T125, G127, L151, and L158 residues. Figures were drawn with PyMOL (Delano Scientific) software using a PDB coordinate file (code 1rlu).

Representative MOHCA cleavage profiles illustrating different classes of “hits” observed in the unfolded state (A), the compact non-native state in high monovalent ion concentrations (B), and the native state (C) of the P4–P6 RNA. Cleavage agents depicted are, from left to right, A115 (5′ radiolabeled RNA), C138 (5′), C222 (3′), and C170 (5′). Two replicates are shown (red and orange) with corresponding background estimates as error bars in blue and cyan, respectively. Statistically significant hits, supported by additional replicates (SI Table 1), are marked with black arrows. Some smaller peaks are also visible but do not pass the stringent statistical criteria used in this work (SI Text).

Pie chart A represents the period from 1958 to 1962; B period from 1971 to 1075; C period from 1985 to 1989; and D period from 2003 to 2007. Each period shows the five major causes of maternal mortality according to international codes of disease. Code homologation was carried out using ICD 7^th version (ICD-7) as reference. Causes of death present in ICD versions 8, 9 and 10 were grouped using ICD-7 codes A115 (Sepsis); A116 (Hypertension, Eclampsia and Toxaemias); A117 (Haemorrhage); A118 and A119 (Abortion); A120 (Other direct and indirect obstetric causes of death, including ectopic pregnancy, hydatidiform mole and other abnormal products of conceptions). Pie charts C and D include a more specific subgroup for death causes from ICD-9 and ICD-10.