PMC

Influence of Lv-si-5b and Lv-si-6 and BACE1 silencing on expression of selected genes in HCN A94 cells, as determined by RT-PCR.

Spore production after three days of development (Fig. 6) varied significantly across nutrient treatments and nearly significantly among strains (One-way ANOVA; F = 76.06, df = 3, p < 0.001 and F = 2.29, df = 5, p = 0.053, respectively) but not among replicate blocks (F = 0.06, df = 3, p = 0.979). No spores were recovered from plates supplemented with 10 g/L tryptone for any strain. The average spore count for all strains did not differ significantly between the 0 g/L and 0.32 g/L tryptone treatments, but was significantly reduced at 3.2 relative to 0.32 g/L tryptone (two-sample, two-sided t-test; df = 17, p < 0.001). DK836 and A94 were the only strains to show significant drops in spore production at 0.32 g/L tryptone relative to zero tryptone (p < 0.025 for DK836 and A94, one-sided t-tests, df = 3). Drops in spore production at 3.2 g/L tryptone (relative to lower tryptone levels) were relatively small for strains DK836, A66 and A75 but were very large for GJV1 (>1000-fold decrease relative to 0.32 g/L tryptone), A45 and A94 (complete loss of spore production at 3.2 g/L tryptone in both cases).

Restoration of Bcl2 expression in A94 cells protects against ER stress-induced death. A94 cells transfected with bcl2 expression constructs show greatly enhanced Bcl2 levels (Western blot, insert). Following treatment with either thapsigargin (1 μM, 24 h) or tunicamycin (1 μg/ml, 24 h), cells were harvested, stained with PI, and analyzed using flow cytometry. The sub-G₁ content of each cell population was quantified. Data are averages of at least three independent experiments and error bars represent standard errors of the means.

Interaction between CPD photoreactivation and homologous recombination. White bars represent the photolyase mutant line A94 (uvr2–1/GUS) and black bars indicate the corresponding wild-type line A87 (UVR2–1/GUS). Recombination frequencies were determined in both lines after irradiation as described in Fig. 2.