Apc2 Adenomatous polyposis coli 2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Apc2provided by FlyBase
Official Full Name: Adenomatous polyposis coli 2provided by FlyBase
Primary source: FLYBASE:FBgn0026598
Locus tag: Dmel_CG6193
See related: AllianceGenome:FB:FBgn0026598
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: APC; apc2; APC2; CG6193; d-APC2; D-APC2; dapc2; dApc2; dAPC2; dAPC2/E-APC; Dm APC2; Dmel\CG6193; e-apc; E-APC; E-APC dAPC2
Summary: Enables beta-catenin binding activity and molecular adaptor activity. Involved in several processes, including embryonic development via the syncytial blastoderm; oogenesis; and regionalization. Located in several cellular components, including apical cortex; basal cortex; and cleavage furrow. Part of beta-catenin destruction complex. Colocalizes with actin filament. Is expressed in several structures, including embryonic/larval central nervous system; epithelium; gastrula embryo; germline stem cell; and imaginal disc. Human ortholog(s) of this gene implicated in several diseases, including Sotos syndrome 3; carcinoma (multiple); complex cortical dysplasia with other brain malformations; familial adenomatous polyposis 1; and hereditary desmoid disease. Orthologous to human APC (APC regulator of WNT signaling pathway) and APC2 (APC regulator of WNT signaling pathway 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

Location:: 95E6-95E6; 3-83 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (24163966..24168264)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (19989688..19993485)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

mitochondrial energy production, Nrg/Ank2, branched actin generated by Arp2/3 and Fz/G proteins/Axin function as four modules that control localization of the microtubule regulator Apc2 to its site of action in dendrite branch points.
Title: Identification of Proteins Required for Precise Positioning of Apc2 in Dendrites.
Data suggest that brain tumor protein (Brat) promotes midline crossing through directing the localization or stability of Adenomatous polyposis coli-2 (Apc2) at the plus ends of microtubules in navigating commissural axons.
Title: Brain Tumor promotes axon growth across the midline through interactions with the microtubule stabilizing protein Apc2.
This study provides new perspectives on the cellular effects of APC that might lead to a deeper understanding of its role in development.
Title: Proteomic analysis reveals APC-dependent post-translational modifications and identifies a novel regulator of β-catenin.
APC2 self-association is required for WNT destructosome activity in the Drosophila embryo.
Title: Self-association of the APC tumor suppressor is required for the assembly, stability, and activity of the Wnt signaling destruction complex.
Germline specific overexpression of Adenomatous Polyposis Coli 2 rescued germline stem cell loss in chic hypomorphs, suggesting an additive role of APC2 and F-actin in maintaining the adherens junctions that anchor germline stem cells to the niche.
Title: The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells.
APC2 protects genome stability by modulating mitotic fidelity through regulation of the cytoskeleton.
Title: APC2 and Axin promote mitotic fidelity by facilitating centrosome separation and cytoskeletal regulation.
These data suggest that APC2 does not have to shuttle into the nucleus or localize to a particular subcellular location to regulate Wnt signaling.
Title: Regulation of Wnt signaling by the tumor suppressor adenomatous polyposis coli does not require the ability to enter the nucleus or a particular cytoplasmic localization.
Data show that a loss of function for dapc1 or both dapc1 and dapc2 (dapc) in ovary border (BC) and adjacent follicle cells impaired BC delamination, and this mutant phenotype can be rescued upon overexpression of either dapc1 or dapc2.
Title: Drosophila apc regulates delamination of invasive epithelial clusters.
Data show that although cortical localization is required for the APC2 function in organizing actin, cortical localization is dispensable for its role in regulating Wnt signaling.
Title: Cortical localization of APC2 plays a role in actin organization but not in Wnt signaling in Drosophila.
APC2 and DIA function in a novel complex that affects actin directly, rather than through an effect on microtubules
Title: A novel role for an APC2-Diaphanous complex in regulating actin organization in Drosophila.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables beta-catenin binding	IBA Inferred from Biological aspect of Ancestor more info
enables beta-catenin binding	IEA Inferred from Electronic Annotation more info
enables beta-catenin binding	IPI Inferred from Physical Interaction more info	PubMed
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in brain development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell fate specification	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in chitin-based embryonic cuticle biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chitin-based larval cuticle pattern formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in chitin-based larval cuticle pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cortical actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of microtubule cytoskeleton polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in follicle cell of egg chamber development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in imaginal disc pattern formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of canonical Wnt signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of canonical Wnt signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of canonical Wnt signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of microtubule depolymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nervous system development	IBA Inferred from Biological aspect of Ancestor more info
involved_in nervous system development	IDA Inferred from Direct Assay more info	PubMed
involved_in neuroblast proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in oocyte localization involved in germarium-derived egg chamber formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in oocyte localization involved in germarium-derived egg chamber formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pattern specification process	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pseudocleavage involved in syncytial blastoderm formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in segment polarity determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in syncytial nuclear migration	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with actin filament	IDA Inferred from Direct Assay more info	PubMed
located_in apical cortex	IDA Inferred from Direct Assay more info	PubMed
located_in basal cortex	IDA Inferred from Direct Assay more info	PubMed
part_of beta-catenin destruction complex	IBA Inferred from Biological aspect of Ancestor more info
part_of beta-catenin destruction complex	IDA Inferred from Direct Assay more info	PubMed
part_of beta-catenin destruction complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of beta-catenin destruction complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of catenin complex	IBA Inferred from Biological aspect of Ancestor more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cleavage furrow	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with cytoplasmic microtubule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: adenomatous polyposis coli 2

Names: Apc2-PA; Apc2-PB; Apc2-PC; CG6193-PA; CG6193-PB; CG6193-PC; adenomatous polyposis coli tumor suppressor homolog 2; epithelial adenomatous polyposis coli

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

24163966..24168264

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001347815.1 → NP_001334699.1 adenomatous polyposis coli 2, isoform C [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

Q961B0, Q9Y1T2
NM_001347814.1 → NP_001334698.1 adenomatous polyposis coli 2, isoform B [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1Z1CN92, Q961B0
NM_058082.3 → NP_477430.1 adenomatous polyposis coli 2, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477430.1

Status: REVIEWED

UniProtKB/TrEMBL

Q961B0, Q9Y1T2

Related

FBpp0083937

Conserved Domains (4) summary

cd00020
Location:281 → 400

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

pfam05923
Location:737 → 761

APC_crr; APC cysteine-rich region

pfam05972
Location:497 → 511

APC_15aa; APC 15 residue motif

sd00043
Location:273 → 314

ARM; armadillo repeat [structural motif]