grpE nucleotide exchange factor GrpE [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: grpE
Gene description: nucleotide exchange factor GrpE
Primary source: ASAP:ABE-0008598
Locus tag: b2614
See related: ECOCYC:EG10416
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2610
Summary: HT_Cmplx22_Cyt: [More information is available at EcoGene: EG10416]. Review: [More information is available at EcoCyc: EG10416].
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Genomic context

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Sequence:: NC_000913.3 (2750115..2750708, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The interaction of DnaK with DnaJ, GrpE, or sigma32 becomes weaker when DnaK is glutathionylated during oxidative stress, and the interaction is restored upon deglutathionylation.
Title: Glutathionylation of the Bacterial Hsp70 Chaperone DnaK Provides a Link between Oxidative Stress and the Heat Shock Response.
analysis of DnaJ-DnaK-GrpE chaperone cooperation
Title: Action of the Hsp70 chaperone system observed with single proteins.
The nucleotide exchange factor GrpE modulates the chaperone DnaK allosterism.
Title: Modulation of the chaperone DnaK allosterism by the nucleotide exchange factor GrpE.
GrpE, which also interacts with subdomains IB and IIB, inhibited the interaction between ClpB and DnaK in vitro, suggesting competition between ClpB and GrpE for binding DnaK.
Title: Interplay between E. coli DnaK, ClpB and GrpE during protein disaggregation.
The results presented here suggest overall low protein stability for GrpE1-112 protein and the folding pathway of this tetramer proceeds via a two step mechanism involving a relatively fast phase forming a multimeric intermediate followed by a slow step to the final folded tetramer.
Title: Probing the stability and mechanism for folding of the GrpE1-112 tetrameric deletion mutant of the GrpE protein from E. coli.
The ratio of GrpE, but not DnaJ, to DnaK is crucial for functionality of the DnaK chaperone system and cell-division machinery in vivo and in vitro
Title: The proper ratio of GrpE to DnaK is important for protein quality control by the DnaK-DnaJ-GrpE chaperone system and for cell division.
N-terminal half of GrpE coils (residues 34-68) interacts with DnaK interdomain linker, regulates the nucleotide exchange activity of the cochaperone and is required to stabilize DnaK-substrate complexes in the ADP-bound conformation
Title: GrpE N-terminal domain contributes to the interaction with Dnak and modulates the dynamics of the chaperone substrate binding domain.
analysis of how the water mediated electrostatic interaction gives thermal stability to the "tail" region of the GrpE protein from E. coli
Title: A water mediated electrostatic interaction gives thermal stability to the "tail" region of the GrpE protein from E. coli.
DnaK-DnaJ-GrpE chaperone system has a role in activating the pi initiator protein
Title: The DnaK-DnaJ-GrpE chaperone system activates inert wild type pi initiator protein of R6K into a form active in replication initiation.
Data describe the trigger factor concentration dependence of the glyceraldehyde-3-phosphate dehydrogenase reactivation yield in the presence and absence of the DnaK-DnaJ-GrpE chaperone system in vitro.
Title: Dimeric trigger factor stably binds folding-competent intermediates and cooperates with the DnaK-DnaJ-GrpE chaperone system to allow refolding.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables adenyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables adenyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables adenyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein-folding chaperone binding	IEA Inferred from Electronic Annotation more info
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within chaperone cofactor-dependent protein refolding	IDA Inferred from Direct Assay more info	PubMed
involved_in protein folding	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein unfolding	IDA Inferred from Direct Assay more info	PubMed
involved_in protein-containing complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to heat	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to heat	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: nucleotide exchange factor GrpE

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2750115..2750708 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417104.1 nucleotide exchange factor GrpE [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417104.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A7D7HYN4

Conserved Domains (2) summary

PRK10325
Location:1 → 197

PRK10325; heat shock protein GrpE; Provisional

cd00446
Location:59 → 106

GrpE; 2-helical coiled coil [structural motif]