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Binding affinity to S1PR4 (unknown origin) by SPR assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Displacement of [32P]S1P from human recombinant S1PR4 incubated for 60 mins by competitive binding assay based scintillation counter
Assay data:1 Active, 8 Activity ≤ 1 µM, 8 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to S1PR4 (unknown origin) assessed as dissociation constant by surface plasmon resonance analysis
Assay data:8 Activity ≤ 1 µM, 8 Tested
SummaryCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Antagonist activity at S1P4 receptor (unknown origin) assessed as equilibrium binding constant by [35S]GTPgammaS binding assay
Assay data:1 Active, 1 Activity ≤ 1 nM, 1 Activity ≤ 1 µM, 1 Tested
Competition Binding Assay from US Patent US11034691: "Sphinogosine-1 -phosphate receptor modulators for treatment of cardiopulmonary disorders"
Assay data:355 Active, 260 Activity ≤ 1 µM, 366 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
In Vitro Binding Assay from US Patent US10676467: "Compositions for binding sphingosine-1-phosphate receptor 1 (S1P1), imaging of S1P1, and methods of use thereof"
Assay data:55 Active, 1 Activity ≤ 1 nM, 58 Activity ≤ 1 µM, 58 Tested
TBD from US Patent US10323029: "Sphinogosine-1-phosphate receptor modulators for treatment of cardiopulmonary disorders"
Assay data:354 Active, 260 Activity ≤ 1 µM, 365 Tested
[32P] S1P Binding Assay from Article 10.1016/j.bmc.2004.10.008: "Asymmetric synthesis and biological evaluation of the enantiomeric isomers of the immunosuppressive FTY720-phosphate."
Assay data:4 Active, 1 Activity ≤ 1 nM, 4 Activity ≤ 1 µM, 4 Tested
S1P Receptor Binding Assay from Article 10.1021/jm0503244: "Discovery of potent 3,5-diphenyl-1,2,4-oxadiazole sphingosine-1-phosphate-1 (S1P1) receptor agonists with exceptional selectivity against S1P2 and S1P3."
Assay data:20 Active, 6 Activity ≤ 1 nM, 20 Activity ≤ 1 µM, 20 Tested
PRESTO-Tango GPCRome screening (S1PR4)
SummaryRelated BioAssays by Target
Inhibition of S1P4 (unknown origin)
Agonist activity at human recombinant S1P4 expressed in CHO cell membranes assessed as stimulation of [35S]-GTPgammaS binding measured after 45 mins by liquid scintillation counting assay
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
Agonist activity at recombinant human S1P4 expressed in CHO cell membranes assessed as stimulation of [35S]-GTPgammaS binding measured after 45 mins by liquid scintillation counting method
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Agonist activity at recombinant human S1P4 receptor expressed in Chem-1 cells assessed as calcium flux measured for 180 secs by FLIPR assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Antagonist activity at recombinant human S1P4 receptor expressed in Chem-1 cells assessed as EC80 S1P-induced calcium flux measured for 180 secs by FLIPR assay
Assay data:2 Active, 28 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Immunomodulatory activity in human S1PR4 expressed in CHO-K1 EDG1 beta-arrestin cells assessed as stimulation of beta-arrestin recruitment incubated for 90 mins at 37 degC followed by incubated for 15 mins in room temperature by pathhunter beta-arrestin assay
Assay data:2 Tested
Agonist activity at S1P4 receptor (unknown origin) measured after 45 mins by [35S]GTP-gammaS binding assay relative to control
Assay data:4 Tested
Agonist activity at S1P4 receptor (unknown origin) measured after 45 mins by [35S]GTP-gammaS binding assay
Assay data:2 Active, 2 Activity ≤ 1 nM, 2 Activity ≤ 1 µM, 4 Tested
Displacement of [32P]S1P from recombinant human S1PR4 expressed in commercial cell membranes incubated for 60 mins by scintillation counting method
Assay data:6 Activity ≤ 1 µM, 8 Tested
Displacement of [33P]-S1P from human S1P4 receptor expressed in CHO-K1 cells after 60 mins by scintillation counting method
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