Entry - *616290 - ZINC FINGER PROTEIN 658; ZNF658 - OMIM

 
 
* 616290

ZINC FINGER PROTEIN 658; ZNF658


HGNC Approved Gene Symbol: ZNF658

Cytogenetic location: 9q21.11     Genomic coordinates (GRCh38): 9:66,900,725-66,932,141 (from NCBI)


TEXT

▼ Description

ZNF658 is a zinc-dependent transcriptional regulator that binds to palindromic DNA that forms a zinc transcriptional regulatory element (ZTRE) in the promoter region of genes involved in zinc homeostasis. ZNF658 also appears to regulate rRNA biogenesis, a highly zinc-dependent process, through clustered ZTREs in the 45S rRNA precursor (see 180450) (summary by Ogo et al., 2015).


▼ Cloning and Expression

Using mass spectrometric analysis to identify proteins in human CaCo-2 colon carcinoma cells that bound the ZTRE in the promoter region of the zinc transporter SLC30A5 (607819), Ogo et al. (2015) identified ZNF658. The deduced 1,059-amino acid protein has an N-terminal Kruppel-associated box (KRAB) domain, followed by 21 central and C-terminal zinc fingers.


▼ Gene Function

Ogo et al. (2015) found that siRNA-mediated knockdown of ZNF658 in CaCo-2 cells increased mRNA expression of 3 zinc-dependent genes, SLC30A5, SLC30A10 (611146), and CBWD (see 611078), and reduced their sensitivity to elevated zinc concentration. EMSA revealed that ZNF658 directly bound the ZTRE within the SLC30A5 promoter region. Zinc fingers 12 to 15 of ZNF658 and both sides of the ZTRE were required for binding. Microarray analysis revealed that knockdown of ZNF658 also elevated expression of several ribosomal protein genes, but reduced the abundance of several rRNAs that arise from the 45S rRNA precursor. Sequencing revealed the presence of clustered ZTREs within the 45S rRNA precursor. Ogo et al. (2015) concluded that ZNF658 has a dual role in repressing expression of genes involved in zinc homeostasis and in regulating rRNA processing.


▼ Mapping

Hartz (2015) mapped the ZNF658 gene to chromosome 9q21.11 based on an alignment of the ZNF658 sequence (GenBank AK024222) with the genomic sequence (GRCh38).

▼ REFERENCES

  1. Hartz, P. A. Personal Communication. Baltimore, Md. 3/31/2015.

  2. Ogo, O. A., Tyson, J., Cockell, S. J., Howard, A., Valentine, R. A., Ford, D. The zinc finger protein ZNF658 regulates the transcription of genes involved in zinc homeostasis and affects ribosome biogenesis through the zinc transcriptional regulatory element. Molec. Cell. Biol. 35: 977-987, 2015. [PubMed: 25582195, images, related citations] [Full Text]


Creation Date:
Patricia A. Hartz : 3/31/2015
Edit History:
carol : 03/31/2015
mcolton : 3/31/2015

* 616290

ZINC FINGER PROTEIN 658; ZNF658


HGNC Approved Gene Symbol: ZNF658

Cytogenetic location: 9q21.11     Genomic coordinates (GRCh38): 9:66,900,725-66,932,141 (from NCBI)


TEXT

Description

ZNF658 is a zinc-dependent transcriptional regulator that binds to palindromic DNA that forms a zinc transcriptional regulatory element (ZTRE) in the promoter region of genes involved in zinc homeostasis. ZNF658 also appears to regulate rRNA biogenesis, a highly zinc-dependent process, through clustered ZTREs in the 45S rRNA precursor (see 180450) (summary by Ogo et al., 2015).


Cloning and Expression

Using mass spectrometric analysis to identify proteins in human CaCo-2 colon carcinoma cells that bound the ZTRE in the promoter region of the zinc transporter SLC30A5 (607819), Ogo et al. (2015) identified ZNF658. The deduced 1,059-amino acid protein has an N-terminal Kruppel-associated box (KRAB) domain, followed by 21 central and C-terminal zinc fingers.


Gene Function

Ogo et al. (2015) found that siRNA-mediated knockdown of ZNF658 in CaCo-2 cells increased mRNA expression of 3 zinc-dependent genes, SLC30A5, SLC30A10 (611146), and CBWD (see 611078), and reduced their sensitivity to elevated zinc concentration. EMSA revealed that ZNF658 directly bound the ZTRE within the SLC30A5 promoter region. Zinc fingers 12 to 15 of ZNF658 and both sides of the ZTRE were required for binding. Microarray analysis revealed that knockdown of ZNF658 also elevated expression of several ribosomal protein genes, but reduced the abundance of several rRNAs that arise from the 45S rRNA precursor. Sequencing revealed the presence of clustered ZTREs within the 45S rRNA precursor. Ogo et al. (2015) concluded that ZNF658 has a dual role in repressing expression of genes involved in zinc homeostasis and in regulating rRNA processing.


Mapping

Hartz (2015) mapped the ZNF658 gene to chromosome 9q21.11 based on an alignment of the ZNF658 sequence (GenBank AK024222) with the genomic sequence (GRCh38).

REFERENCES

  1. Hartz, P. A. Personal Communication. Baltimore, Md. 3/31/2015.

  2. Ogo, O. A., Tyson, J., Cockell, S. J., Howard, A., Valentine, R. A., Ford, D. The zinc finger protein ZNF658 regulates the transcription of genes involved in zinc homeostasis and affects ribosome biogenesis through the zinc transcriptional regulatory element. Molec. Cell. Biol. 35: 977-987, 2015. [PubMed: 25582195] [Full Text: https://doi.org/10.1128/MCB.01298-14]


Creation Date:
Patricia A. Hartz : 3/31/2015

Edit History:
carol : 03/31/2015
mcolton : 3/31/2015



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OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.

NOTE: OMIM is intended for use primarily by physicians and other professionals concerned with genetic disorders, by genetics researchers, and by advanced students in science and medicine. While the OMIM database is open to the public, users seeking information about a personal medical or genetic condition are urged to consult with a qualified physician for diagnosis and for answers to personal questions.
OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.
Printed: April 25, 2024