Alternative titles; symbols
HGNC Approved Gene Symbol: MIR25
Cytogenetic location: 7q22.1 Genomic coordinates (GRCh38): 7:100,093,560-100,093,643 (from NCBI)
MicroRNAs (miRNAs), such as miRNA25, are 20- to 23-nucleotide RNAs that regulate gene expression posttranscriptionally by mediating sequence-specific repression of mRNA translation (Lagos-Quintana et al., 2001).
Using a directional cloning procedure to identify miRNAs from HeLa cell total RNA, Lagos-Quintana et al. (2001) cloned miRNA25. Northern blot analysis showed expression of miRNA25 in HeLa cells, mouse kidney, and adult fish.
Using microarray analysis, Ciafre et al. (2005) found that expression of MIR25 was significantly upregulated in primary glioblastomas compared with normal peripheral brain tissue.
Petrocca et al. (2008) found that MCM7 (600592) and the precursors of miRNAs MIR106B (612983), MIR93 (612984), and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors. Accumulation of precursor RNA containing MCM7, MIR106B, MIR93, and MIR25 was induced following entry into G1 phase in a synchronized human gastric cancer cell line and was associated with expression of the master cell cycle regulator E2F1 (189971). Overexpression of MIR106B, MIR93, and MIR25 in gastric cancer cells reduced cell response to TGF-beta (TGFB1; 190180) by interfering with synthesis of p21 (CDKN1A; 116899) and BIM (BCL2L11; 603827), the 2 most downstream effectors of TGF-beta-dependent cell cycle arrest and apoptosis, respectively. Petrocca et al. (2008) concluded that the MIR106B-MIR93-MIR25 cluster, which is activated by E2F1 and upregulated in human adenocarcinomas, alters the response of gastric cancer cells to TGF-beta, affecting both cell cycle arrest and apoptosis.
Using high-throughput functional screening of the human microRNAome, Wahlquist et al. (2014) identified miRNAs that suppress intracellular calcium handling in heart muscle by interacting with mRNA encoding the sarcoplasmic reticulum calcium uptake pump SERCA2a (ATP2A2; 108740). Of 875 miRNAs tested, MIR25 potently delayed calcium uptake kinetics in cardiomyocytes in vitro and was upregulated in heart failure, both in mice and humans. Whereas adeno-associated virus 9 (AAV9)-mediated overexpression of MIR25 in vivo resulted in a significant loss of contractile function, injection of an antisense oligonucleotide (antagomir) against MIR25 markedly halted established heart failure in a mouse model, improving cardiac function and survival relative to a control antagomir. Wahlquist et al. (2014) concluded that their data revealed that increased expression of endogenous MIR25 contributes to declining cardiac function during heart failure and suggested that it might targeted therapeutically to restore function.
Ciafre et al. (2005) stated that the MIR25 gene maps to chromosome 7q22, where it lies in a cluster with the MIR106B and MIR93 genes.
Petrocca et al. (2008) reported that the MIR106B, MIR93, and MIR25 genes map to chromosome 7q22 and are clustered in a 5-prime to 3-prime orientation within intron 13 of the MCM7 gene.
Ciafre, S. A., Galardi, S., Mangiola, A., Ferracin, M., Liu, C.-G., Sabatino, G., Negrini, M., Maira, G., Croce, C. M., Farace, M. G. Extensive modulation of a set of microRNAs in primary glioblastoma. Biochem. Biophys. Res. Commun. 334: 1351-1358, 2005. [PubMed: 16039986] [Full Text: https://doi.org/10.1016/j.bbrc.2005.07.030]
Lagos-Quintana, M., Rauhut, R., Lendeckel, W., Tuschl, T. Identification of novel genes coding for small expressed RNAs. Science 294: 853-858, 2001. [PubMed: 11679670] [Full Text: https://doi.org/10.1126/science.1064921]
Petrocca, F., Visone, R., Onelli, M. R., Shah, M. H., Nicoloso, M. S., de Martino, I., Iliopoulos, D., Pilozzi, E., Liu, C.-G., Negrini, M., Cavazzini, L., Volinia, S., Alder, H., Ruco, L. P., Baldassarre, G., Croce, C. M., Vecchione, A. E2F1-regulated microRNAs impair TGF-beta-dependent cell-cycle arrest and apoptosis in gastric cancer. Cancer Cell 13: 272-286, 2008. [PubMed: 18328430] [Full Text: https://doi.org/10.1016/j.ccr.2008.02.013]
Wahlquist, C., Jeong, D., Rojas-Munoz, A., Kho, C., Lee, A., Mitsuyama, S., van Mil, A., Park, W. J., Sluijter, J. P. G., Doevendans, P. A. F., Hajjar, R. J., Mercola, M. Inhibition of miR-25 improves cardiac contractility in the failing heart. Nature 508: 531-535, 2014. [PubMed: 24670661] [Full Text: https://doi.org/10.1038/nature13073]