Alternative titles; symbols
HGNC Approved Gene Symbol: L3MBTL2
Cytogenetic location: 22q13.2 Genomic coordinates (GRCh38): 22:41,205,312-41,231,271 (from NCBI)
By database analysis using the mbt repeats of Drosophila l(3)mbt as probe, Wismar (2001) identified L3MBTL2. The 706-amino acid protein has an N-terminal C2-C2 zinc finger and 4 mbt repeats. Northern blot analysis detected a 3.6-kb transcript in all human tissues examined with highest expression in brain and pancreas, followed by heart, placenta, liver, skeletal muscle, and kidney, with low expression in lung. A 3.8-kb transcript, which is due to inclusion of exon 15, was detected in EST clones from fetal liver and spleen. The splice variant results in a frameshift and premature termination at amino acid 615.
By yeast 2-hybrid analysis with c-Myb (MYB; 189990) as bait, followed by screening an adult mouse brain cDNA library, Markus et al. (2003) cloned mouse L3mbtl2. Human and mouse L3MBTL2 share 90.8% amino acid identity. Northern blot analysis of mouse tissues detected strong expression in testes, moderate expression in lung, kidney, brain, and thymus, and weak expression in liver, heart, spleen, intestine, and bone marrow. Expression was detected in immature hematopoietic cell lines of myeloid, erythroid, and lymphoid origin, as well as before embryonic day 7 through all stages of embryonic development. Western blot, phosphorylation, and immunoprecipitation studies demonstrated that L3mbtl2 is phosphorylated. Pulse-chase studies identified L3mbtl2 as a relatively unstable protein with an estimated half-life of approximately 4.7 hours. Fluorescence microscopy localized L3mbtl2 to the nucleus.
Markus et al. (2003) observed a rapid decrease in L3mbtl2 expression at the late stage of monocytic differentiation in connection with growth arrest. High L3mbtl2 expression in NIH3T3 cells declined in contact-inhibited cells and also declined after serum starvation. Expression increased after serum stimulation of NIH3T3 cells upon reentry into the cell cycle. Markus et al. (2003) suggested that L3mbtl2 plays a role in cell proliferation or differentiation.
Wismar (2001) determined that the L3MBTL2 gene contains 18 exons.
By genomic sequence analysis, Wismar (2001) mapped the L3MBTL2 gene to chromosome 22q13.3.
By genomic sequence analysis, Markus et al. (2003) mapped the mouse L3mbtl2 gene to chromosome 15E2.
Markus, J., Feikova, S., Sramko, M., Wolff, L., Bies, J. Proliferation-linked expression of the novel murine gene m4mbt encoding a nuclear zinc finger protein with four mbt domains. Gene 319: 117-126, 2003. [PubMed: 14597177] [Full Text: https://doi.org/10.1016/s0378-1119(03)00801-1]
Wismar, J. Molecular characterization of h-l(3)mbt-like: a new member of the human mbt family. FEBS Lett. 507: 119-121, 2001. [PubMed: 11682070] [Full Text: https://doi.org/10.1016/s0014-5793(01)02959-3]