16S v3v4 of Extraction Kit Negative Control - SRA

SRX9517111: 16S v3v4 of Extraction Kit Negative Control
1 ILLUMINA (Illumina MiSeq) run: 6,054 spots, 3.6M bases, 2.6Mb downloads

Design: Negative controls of the Qiagen PowerFecal DNA. Eluted with 100 l of ddH2O pre-warmed to 60C. Prior to sequencing, we quantified the DNA in eluted extracts using a Qubit dsDNA BR Assay Kit and standardized DNA concentration to 20 ng/uL prior to PCR amplification. We PCR amplified the v3v4 region of the 16S rRNA gene using the 341f forward and 805r reverse universal primers. PCR products were sequenced on an Illumina MiSeq platform (v3 chemistry: 2 x 300 base-pair read pairs) at the University of Calgary Centre for Health Genomics and Informatics.

Submitted by: University of Calgary

Study: Sable Island horse fecal bacterial microbiome 16S sequences

PRJNA674675 • SRP291220 • All experiments • All runs

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Bacterial 16S amplicon sequences generated from fecal samples collected from 86 adult mares from a population of feral horses on Sable Island Nova Scotia. The objective of the study was to characterize the Sable Island horse fecal microbiome and to parse the effects of local environmental and spatial processes.

Sample: Negative Control

SAMN16793950 • SRS7724874 • All experiments • All runs

Organism: metagenome

Library:

Name: Si_2014_16S_Blank2

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 6,054 spots, 3.6M bases, 2.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR13069593	6,054	3.6M	2.6Mb	2020-12-17

ID:: 12436899

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